Nanosecond bacteria inactivation realized by locally enhanced electric field treatment
Nanosecond bacteria inactivation realized by locally enhanced electric field treatment
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DOI：
10.1038/s44221-022-00003-2
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发表时间：
2023-01
期刊：
影响因子：
--
通讯作者：
Ting Wang;Xing Xie
中科院分区：
文献类型：
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作者：
Ting Wang;Xing Xie
水体中的细菌污染仍然是威胁公众健康的重要因素，寻求有效的水消毒方法具有重要意义。在这里，我们表明，局部增强电场处理（LEEFT）的纳米级尖端结构修饰的电极可以诱导超快细菌灭活纳秒电脉冲。一个芯片上的实验室设备上的电极上的金镶嵌边缘开发的operando调查。由于避雷针效应，电穿孔可使边缘尖端的细菌失活。在55 kV cm− 1下的单个20 ns脉冲可实现26.6%的细菌灭活，在40 kV cm− 1下的10个脉冲可实现95.1%的灭活。LEEFT与不带边缘的系统相比，可将外加电场降低约8倍，或将处理时间缩短至少106倍。革兰氏阳性菌和革兰氏阴性菌，包括耐药菌，都被LEEFT用纳秒脉冲灭活。根据模拟，当位于边缘尖端的细胞膜被尖端处的集中电荷直接充电时，其充电快得多并且达到高得多的水平，导致瞬时电穿孔和细胞失活。
Bacterial contamination in water is still a critical threat to public health; seeking efficient water disinfection approaches is of great significance. Here we show that locally enhanced electric field treatment (LEEFT) by electrodes modified with nanoscale tip structures can induce ultrafast bacteria inactivation with nanosecond electrical pulses. A lab-on-a-chip device with gold nanowedges on the electrodes is developed for an operando investigation. Attributed to the lightning-rod effect, the bacteria at the nanowedge tips are inactivated by electroporation. A single 20 ns pulse at 55 kV cm−1has achieved 26.6% bacteria inactivation, with ten pulses at 40 kV cm−1resulting in 95.1% inactivation. LEEFT lowers the applied electric field by about 8 fold or shortens the treatment time by at least 106fold, compared with the system without nanowedges. Both Gram-positive and Gram-negative bacteria, including antibiotic-resistant bacteria, are inactivated with nanosecond pulses by LEEFT. According to simulation, when the membrane of the cell located at the nanowedge tip is directly charged by the concentrated charges at the tip, it is charged much faster and to a much higher level, leading to instant electroporation and cell inactivation.