Determination of Rigidity of Protein Bound Au144 Clusters by Electron Cryomicroscopy
Determination of Rigidity of Protein Bound Au144 Clusters by Electron Cryomicroscopy
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DOI：
10.1021/jp101970x
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发表时间：
2010-09-30
影响因子：
3.7
通讯作者：
Ackerson, Christopher J.
中科院分区：
文献类型：
--
作者：
Sexton, Jonathan Z.;Ackerson, Christopher J.
提出了一种估算蛋白质结合金纳米颗粒位置位移的方法，并用于估算Au-144纳米颗粒与四聚体模型蛋白质结合的连接刚性。我们观察到位移值的分布，其中大多数Au-144簇相对于蛋白质质心固定在3埃内。分布的形状表明热运动和蛋白质变形两个物理过程。这种和类似的刚性金纳米粒子/蛋白质共轭物在高分辨率单粒子电子冷冻显微镜的应用进行了讨论。
A method for estimating the positional displacement of protein bound gold nanoparticles is presented and used to estimate the rigidity of linkage of Au-144 nanoparticles bound to a tetrameric model protein. We observe a distribution of displacement values where most Au-144 clusters are immobilized to within 3 angstrom relative to the protein center of mass. The shape of the distribution suggests two physical processes of thermal motion and protein deformation. The application of this and similar rigid gold nanoparticle/protein conjugates in high resolution single particle electron cryo-microscopy is discussed.