Identification of a shootin1 isoform expressed in peripheral tissues
Identification of a shootin1 isoform expressed in peripheral tissues
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DOI：
10.1007/s00441-016-2415-9
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发表时间：
2016-10-01
影响因子：
3.6
通讯作者：
Inagaki, Naoyuki
中科院分区：
文献类型：
--
作者：
Higashiguchi, Yasuna;Katsuta, Kazuhiro;Inagaki, Naoyuki
Shootin 1是一种脑特异性胞质蛋白，参与神经元极性形成和轴突生长。它聚集在轴突生长锥的前缘，在那里它作为离合器分子介导F-肌动蛋白逆行流动和细胞粘附之间的机械耦合，从而产生轴突生长的力。在这项研究中，我们报告了一种新的剪接异构体shootin 1不仅在大脑中，而且在外周组织中表达。我们将脑特异性shootin 1重命名为shootin 1a，并将新的亚型命名为shootin 1b。免疫印迹和免疫组织化学分析与shootin 1b特异性抗体显示，shootin 1b分布在各种小鼠组织，包括肺，肝，胃，肠，脾，胰腺，肾和皮肤。有趣的是，shootin 1b免疫反应被广泛检测到上皮细胞，构成简单的和复层上皮;在一些细胞中，它与E-钙粘蛋白和coronarin在细胞间接触部位共定位。Shootin 1b还定位于脾脏中的树突状细胞。这些结果表明shootin 1b可能在包括上皮细胞在内的多种外周组织中发挥作用。
Shootin1 is a brain-specific cytoplasmic protein involved in neuronal polarity formation and axon outgrowth. It accumulates at the leading edge of axonal growth cones, where it mediates the mechanical coupling between F-actin retrograde flow and cell adhesions as a clutch molecule, thereby producing force for axon outgrowth. In this study, we report a novel splicing isoform of shootin1 which is expressed not only in the brain but also in peripheral tissues. We have renamed the brain-specific shootin1 as shootin1a and termed the novel isoform as shootin1b. Immunoblot and immunohistochemical analyses with a shootin1b-specific antibody revealed that shootin1b is distributed in various mouse tissues including the lung, liver, stomach, intestines, spleen, pancreas, kidney and skin. Interestingly, shootin1b immunoreactivity was widely detected in epithelial cells that constitute simple and stratified epithelia; in some cells, it colocalized with E-cadherin and cortactin at cell-cell contact sites. Shootin1b also localized in dendritic cells in the spleen. These results suggest that shootin1b may function in various peripheral tissues including epithelial cells.