DNA Methylation Profiles of Vegans and Non-Vegetarians in the Adventist Health Study-2 Cohort.
DNA Methylation Profiles of Vegans and Non-Vegetarians in the Adventist Health Study-2 Cohort.
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DOI：
10.3390/nu12123697
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发表时间：
2020-11-30
期刊：
影响因子：
5.9
通讯作者：
Fraser GE
中科院分区：
文献类型：
--
作者：
Miles FL;Mashchak A;Filippov V;Orlich MJ;Duerksen-Hughes P;Chen X;Wang C;Siegmund K;Fraser GE
在基督复临安息日健康研究-2队列中，我们试图确定纯素食者和非素食者之间的DNA甲基化模式是否不同。来自巴菲外套的全基因组DNA甲基化在62名纯素食者和142名非素食者中进行了分析。利用线性回归方法，根据不同的基因/基因间隔区和CpG岛相关区域以及启动子对CpG位点和基因的甲基化进行分类或总结。基因的甲基化被测量为注释到相应基因指定区域的可用CpG的平均甲基化。一种定义零分布的排列方法，改编自Storey等人。是用来调整错误发现的。在区域和总体分析中，几个CpG位点和基因甲基化的差异被检测到错误发现率为0.05。素食饮食主要与基因的低甲基化有关，最明显的是甲基转移酶样蛋白1(METTL1)。尽管在目前的研究中检测到了有限数量的差异甲基化特征，但错误的发现方法揭示了存在更大比例的差异甲基化基因和位置，并且可以在更大的样本量下被检测到。我们的发现表明，素食者和非素食者的DNA甲基化略有不同，随着样本的增加，预计会有更多可检测到的显著差异。
We sought to determine if DNA methylation patterns differed between vegans and non-vegetarians in the Adventist Health Study-2 cohort. Genome-wide DNA methylation derived from buffy coat was profiled in 62 vegans and 142 non-vegetarians. Using linear regression, methylation of CpG sites and genes was categorized or summarized according to various genic/intergenic regions and CpG island-related regions, as well as the promoter. Methylation of genes was measured as the average methylation of available CpG’s annotated to the nominated region of the respective gene. A permutation method defining the null distribution adapted from Storey et al. was used to adjust for false discovery. Differences in methylation of several CpG sites and genes were detected at a false discovery rate < 0.05 in region-specific and overall analyses. A vegan diet was associated predominantly with hypomethylation of genes, most notably methyltransferase-like 1 (METTL1). Although a limited number of differentially methylated features were detected in the current study, the false discovery method revealed that a much larger proportion of differentially methylated genes and sites exist, and could be detected with a larger sample size. Our findings suggest modest differences in DNA methylation in vegans and non-vegetarians, with a much greater number of detectable significant differences expected with a larger sample.
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DOI：
10.18632/aging.100718
发表时间：
2015-02
期刊：
Aging
影响因子：
--
作者：
Bacalini MG;Boattini A;Gentilini D;Giampieri E;Pirazzini C;Giuliani C;Fontanesi E;Remondini D;Capri M;Del Rio A;Luiselli D;Vitale G;Mari D;Castellani G;Di Blasio AM;Salvioli S;Franceschi C;Garagnani P
通讯作者：
Garagnani P
DOI：
10.4049/jimmunol.181.4.2330
发表时间：
2008-08-15
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Coleman AM;Rendon BE;Zhao M;Qian MW;Bucala R;Xin D;Mitchell RA
通讯作者：
Mitchell RA
影响因子：
50.3
作者：
Figueroa ME;Abdel-Wahab O;Lu C;Ward PS;Patel J;Shih A;Li Y;Bhagwat N;Vasanthakumar A;Fernandez HF;Tallman MS;Sun Z;Wolniak K;Peeters JK;Liu W;Choe SE;Fantin VR;Paietta E;Löwenberg B;Licht JD;Godley LA;Delwel R;Valk PJ;Thompson CB;Levine RL;Melnick A
通讯作者：
Melnick A
影响因子：
5.9
作者：
Alshahrani, Saeed Mastour;Fraser, Gary E.;Orlich, Michael J.
通讯作者：
Orlich, Michael J.
影响因子：
6.1
作者：
Yang CS;Fang M;Lambert JD;Yan P;Huang TH
通讯作者：
Huang TH