"Barcode" cell sensor microfluidic system: Rapid and sample-to-answer antimicrobial susceptibility testing applicable in resource-limited conditions
"Barcode" cell sensor microfluidic system: Rapid and sample-to-answer antimicrobial susceptibility testing applicable in resource-limited conditions
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“条形码”细胞传感器微流体系统：适用于资源有限条件下的快速、从样本到答案的抗菌药物敏感性测试
DOI：
10.1016/j.bios.2021.113516
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发表时间：
2021-07-27
影响因子：
12.6
通讯作者：
Ren, Kangning
中科院分区：
文献类型：
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作者：
Chan, Chiu-Wing;Sun, Han;Ren, Kangning
许多快速药敏试验（AST）方法已被提出，以控制临床抗微生物药物耐药性（AMR）并保持剩余抗微生物药物的有效性。然而，在资源有限的条件下检测抗菌素耐药性的方法要少得多，例如对水/食品/公共设施进行频繁的安全筛查，在大流行期间对大量样本进行紧急调查，或在低收入国家进行抗菌素耐药性检测。迄今为止实现的快速AST方法在用于此类调查时存在各种缺点，例如，高成本和需要昂贵的仪器（如显微镜）。更合理的策略是首先通过现场测试筛选样本，然后将任何怀疑含有AMR细菌的样本送去进行高级测试。因此，需要一种具有成本效益的AST，它可以快速处理大量样品而无需使用昂贵的设备。为此，目前的工作展示了一种基于自适应线性滤波器阵列的新型“条形码”细胞传感器，作为一种全自动和无显微镜的方法，用于计数非常小体积的细胞（类似于1.00 x 10(4)个未预孵卵的细胞），其中悬浮细胞浓缩成长度与细胞数量成正比的微棒。我们将这种传感器与芯片上培养方法相结合，我们已经证明了快速和自动化的药物暴露，并实现了一个低成本和资源独立的便携式AST平台，从该平台可以简单地通过手机获得结果。该方法的周转时间（2-3小时）比标准方法（16-24小时）短得多。由于其无需显微镜分析、价格合理、便携、高通量和用户友好性，我们的“条形码”AST系统有可能在没有先进设备的情况下满足AST的各种需求，使其成为对抗AMR的有前途的新工具。
Many rapid antimicrobial susceptibility testing (AST) methods have been proposed to contain clinical antimicrobial resistance (AMR) and preserve the effectiveness of remaining antimicrobials. However, far fewer methods have been proposed to test AMR in resource-limited conditions, such as for frequent safety screenings of water/food/public facilities, urgent surveys of massive samples during a pandemic, or AMR tests in low-income countries. Rapid AST methods realized thus far have a variety of drawbacks when used for such surveys, e.g., high cost and the requirement of expensive instruments such as microscopy. A more reasonable strategy would be to screen samples via onsite testing first, and then send any sample suspected to contain AMR bacteria for advanced testing. Accordingly, a cost-efficient AST is demanded, which can rapidly process a large number of samples without using expensive equipment. To this end, current work demonstrates a novel "barcode" cell sensor based on an adaptive linear filter array as a fully automatic and microscope-free method for counting very small volumes of cells (similar to 1.00 x 10(4) cells without pre-incubation), wherein suspended cells concentrate into microbars with length proportional to the number of cells. We combined this sensor with an on-chip culture approach we had demonstrated for rapid and automated drug exposure and realized a low-cost and resourceindependent platform for portable AST, from which results can be obtained simply through a cell phone. This method has a much shorter turnaround time (2-3 h) than that of standard methods (16-24 h). Thanks to its microscopy-free analysis, affordability, portability, high throughput, and user-friendliness, our "barcode" AST system has the potential to fulfill the various demands of AST when advanced facilities are not available, making it a promising new tool in the fight against AMR.