The expression of inhibin beta B is high in human adipocytes, reduced by weight loss, and correlates to factors implicated in metabolic disease
The expression of inhibin beta B is high in human adipocytes, reduced by weight loss, and correlates to factors implicated in metabolic disease
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DOI：
10.1016/j.bbrc.2006.04.030
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发表时间：
2006-06-16
影响因子：
3.1
通讯作者：
Carlsson, Lena M. S.
中科院分区：
文献类型：
--
作者：
Sjoholm, Kajsa;Palming, Jenny;Carlsson, Lena M. S.
脂肪组织是产生和分泌脂肪因子的内分泌器官。本研究的目的是鉴定主要在人皮下脂肪细胞中表达的基因。为此，开发了一种算法，并使用来自33种人体组织和细胞类型的DNA微阵列表达谱来选择基因。抑制素β B（INHB B;编码激活素β B亚基），并通过实时PCR和免疫组化证实在脂肪细胞中的高表达。脂肪组织中的INHBB表达被饮食诱导的体重减轻下调（p <0.001）。此外，INHBB表达与总脂肪组织面积（P <0.001）和皮下脂肪组织面积（P <0.01）以及空腹胰岛素（P <0.01）和胆固醇（P <0.05）血清水平呈正相关。总之，INHBB在人脂肪细胞中表达较高，体重减轻和脂肪组织INHBB mRNA水平降低与代谢风险因素相关。这表明脂肪细胞中产生的激活素B可能在代谢综合征中起作用。(c)2006年爱思唯尔公司All rights reserved.
Adipose tissue is an endocrine organ that produces and secretes adipokines. The aim of this study was to identify genes predominantly expressed in human subcutaneous adipocytes. For this purpose, an algorithm was developed and DNA microarray expression profiles from 33 human tissues and cell types were used to select genes. Inhibin beta B (INHBB; coding for the activin beta B subunit) was identified and high expression in adipocytes was confirmed by real-time PCR and immunchistochemistry. INHBB expression in adipose tissue was down regulated by diet-induced weight loss (p < 0.001). Furthermore, INHBB expression was positively correlated to total (P < 0.001) and subcutaneous (p < 0.01) adipose tissue areas and serum levels of fasting insulin (p < 0.01) and cholesterol (p < 0.05). In conclusion, INHBB expression was high in human adipocytes, reduced by weight loss and adipose tissue INHBB mRNA levels correlated to metabolic risk factors. This suggests that activin B produced in adipocytes may play a role in the metabolic syndrome. (c) 2006 Elsevier Inc. All rights reserved.