Sesquiterpene Quinones and related metabolites from Phyllosticta spinarum, a fungal strain endophytic in Platycladus orientalis of the Sonoran Desert
Sesquiterpene Quinones and related metabolites from Phyllosticta spinarum, a fungal strain endophytic in Platycladus orientalis of the Sonoran Desert
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DOI：
10.1021/np070600c
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发表时间：
2008-02-01
影响因子：
5.1
通讯作者：
Gunatilaka, A. A. Leslie
中科院分区：
文献类型：
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作者：
Wijeratne, E. M. Kithsiri;Paranagama, Priyani A.;Gunatilaka, A. A. Leslie
从侧柏内生真菌Phyllosticta Spinarum中分离得到5个新的代谢产物：(+)-(5S，10S)-4‘-羟甲基环氮酮(1)、3-酮基牛磺酸(3)、3-α-羟基牛磺酸(4)、12-羟基牛磺酸(5)、叶松酮(6)和牛磺酸(2)。根据新化合物的一维和二维核磁共振波谱数据和化学转化率确定了它们的结构。在一组由五种癌细胞株组成的小组中，所有化合物都被评估为抑制细胞增殖，只有牛磺酸(2)显示出活性。在基于流式细胞术的检测中，牛磺酸诱导PC-3M和NIH3T3细胞系的凋亡。
Five new metabolites, (+)-(5S,10S)-4'-hydroxymethylcyclozonarone (1), 3-ketotauranin (3), 3 alpha-hydroxytauranin (4), 12-hydroxytauranin (5), and phyllospinarone (6), together with tauranin (2), were isolated from Phyllosticta spinarum, a fungal strain endophytic in Platycladus orientalis. The structures of the new compounds were determined on the basis of their 1D and 2D NMR spectroscopic data and chemical interconversions. All compounds were evaluated for inhibition of cell proliferation in a panel of five cancer cell lines, and only tauranin (2) showed activity. When tested in a flow cytometry-based assay, tauranin induced apoptosis in PC-3M and NIH 3T3 cell lines.