Rapid determination of metolcarb residues in foods using a biomimetic enzyme-linked immunosorbent assay employing a novel molecularly imprinted polymer film as artificial antibody.
Rapid determination of metolcarb residues in foods using a biomimetic enzyme-linked immunosorbent assay employing a novel molecularly imprinted polymer film as artificial antibody.
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DOI：
10.5740/jaoacint.10-387
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发表时间：
2013-03
影响因子：
1.6
通讯作者：
Yiwei Tang;Guo-zhen Fang;Shuo Wang;Jingwei Sun;Kun Qian
中科院分区：
文献类型：
--
作者：
Yiwei Tang;Guo-zhen Fang;Shuo Wang;Jingwei Sun;Kun Qian
以分子印迹聚合物（MIP）膜为抗体模拟物，建立了一种快速竞争直接仿生ELISA（cd-BELISA）检测N-甲基氨基甲酸酯类杀虫剂速灭威的方法。采用本体聚合法在96孔板表面直接合成了MIP膜。结果表明，该印迹膜具有抗体-抗原结合特性和快速吸附能力，特别适用于cd-BELISA的开发。对cd-BELISA的反应条件进行了优化。在最佳条件下，cd-BELISA的灵敏度为17 μ g/L，检测限为0.12 μ g/L。交叉反应性表明，cd-BELISA对速灭威的选择性高于结构相关化合物。该方法用于苹果汁、甘蓝和黄瓜中速灭威的测定，平均回收率为71.5%~ 117.0%。通过HPLC进行结果验证，使用这两种方法获得的数据之间具有良好的相关性（r2 > 0.9562）。因此，建立的cd-BELISA法具有快速检测食品中速灭威的潜力。
A novel and fast competitive direct biomimetic ELISA (cd-BELISA) was developed for determination of the N-methylcarbamate insecticide metolcarb based on a molecularly imprinted polymer (MIP) film as the antibody mimic. The MIP film was directly synthesized on the surface of a 96-well plate by bulk polymerization. The synthesized film was characterized, and the results showed that the imprinted film exhibited antibody-antigen-like binding properties and rapid adsorption ability, which was particularly useful for cd-BELISA development. The cd-BELISA conditions were optimized in detail. Under the optimum conditions, the sensitivity and LOD of the cd-BELISA were found to be 17 and 0.12 microg/L, respectively. Crossreactivity demonstrated that the cd-BELISA had a higher selectivity for metolcarb than for structurally related compounds. The developed method was applied to the determination of metolcarb in spiked apple juice, cabbage, and cucumber, with mean recoveries ranging from 71.5 to 117.0%. Validation of the results was conducted by HPLC with good correlation (r2 > 0.9562) between data obtained using these two methods. Therefore, the developed cd-BELISA has potential for the rapid determination of metolcarb in foods.