Distribution of the tandem repeat sequences and karyotyping in cucumber (Cucumis sativus L.) by fluorescence in situ hybridization
Distribution of the tandem repeat sequences and karyotyping in cucumber (Cucumis sativus L.) by fluorescence in situ hybridization
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DOI：
10.1159/000151320
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发表时间：
2008-01-01
影响因子：
1.7
通讯作者：
Jin, W. W.
中科院分区：
文献类型：
--
作者：
Han, Y. H.;Zhang, Z. H.;Jin, W. W.
我们使用全基因组鸟枪读数分析了黄瓜自交系9930基因组中的重复序列组成。分析表明，卫星DNA序列是黄瓜基因组中最具优势的组成部分。利用荧光原位杂交（FISH）技术研究了黄瓜染色体上串联重复序列（I/II型、III型和IV型）的分布规律。III型和45 S rDNA的FISH信号提供了有用的细胞遗传学标记，其位置和荧光强度允许容易地鉴定所有体细胞中期染色体。构建了显示几个串联重复序列的位置和荧光强度的核型。这一基于FISH的核型的建立为黄瓜分子、遗传和细胞遗传图谱的整合以及最终的基因组测序计划奠定了基础。版权所有（C）2008 S. Karger AG，巴塞尔
We analyzed repeat sequences composition in the genome of cucumber inbred line 9930 using whole-genome shotgun reads. The analysis showed that satellite DNA sequences are the most dominant components in the cucumber genome. The distribution pattern of several tandem repeat sequences (Type I/II, Type III and Type IV) on cucumber chromosomes was visualized using fluorescence in situ hybridization (FISH). The FISH signals of the Type III and 45S rDNA provide useful cytogenetic markers, whose position and fluorescence intensity allow for easy identification of all somatic metaphase chromosomes. A karyotype showing the position and fluorescence intensity of several tandem repeat sequences is constructed. The establishment of this FISH-based karyotype has created the basis for the integration of molecular, genetic and cytogenetic maps in Cucumis sativus and for the ultimate genome sequencing project as well. Copyright (C) 2008 S. Karger AG, Basel