Tissue restricted and stage specific transcription is maintained within 411 nucleotides flanking the 5' end of the chicken alpha-skeletal actin gene.
Tissue restricted and stage specific transcription is maintained within 411 nucleotides flanking the 5' end of the chicken alpha-skeletal actin gene.
复制标题
组织限制性和阶段特异性转录维持在鸡 α-骨骼肌动蛋白基因 5 端侧翼的 411 个核苷酸内。
DOI：
10.1093/nar/14.4.1683
复制
发表时间：
1986
影响因子：
14.9
通讯作者：
Schwartz,RJ
中科院分区：
文献类型：
--
作者：
Grichnik,JM;Bergsma,DJ;Schwartz,RJ
在鸡原代成肌细胞培养物中，α-骨骼actln信息水平已被证明受到严格调控。为了检测肌细胞特异性表达所需的基因元件，将含有鸡α-骨骼肌动蛋白、β-细胞质肌动蛋白和组蛋白H2 b基因的5′-侧翼区的DNA序列与氯霉素乙酰转移酶基因的编码序列连接，并转染到肌源性和非肌源性细胞中。与β-肌动蛋白CAT杂交体相反，α-骨架肌动蛋白CAT构建体在转染的非肌源性细胞中显示出受限的CAT表达。我们发现，在成肌细胞融合过程中，α-骨架肌动蛋白基因5′端侧翼的411个核苷酸片段导致CAT酶活性增加9-15倍，而β-肌动蛋白和组蛋白侧翼序列仅瞬时增加2倍。这些结果表明，α-骨架肌动蛋白基因5′端411 bp内的DNA序列影响其细胞类型和阶段特异性表达。
α-skeletal actln message levels have been shown to be tightly regulated in chicken primary myoblast cultures. To test for gene elements required for muscle cell specific expression, DNA sequences containing the 5′-flanking regions of the chicken α-skeletal actin, β-cytoplasmic actin, and the histone H2b genes were linked to the coding sequences of the chloramphenicol acetyltransferase gene and transfected into myogenic and non-myogenic cells. In contrast to β-actin CAT hybrids, the α-skeletal actin CAT constructions displayed restricted CAT expression in transfected non-myogenic cells. We showed that a 411 nucleotide fragment flanking the 5′ end of of the α-skeletal actin gene was responsible for a 9–15 fold increase in CAT enzymatic activity during myoblast fusion, versus only a transient 2 fold rise for the β-actin and histone flanking sequences. These results indicate that DNA sequences within 411 bp of the 5′ terminus of the α-skeletal actin gene influenced its cell type and stage specific expression.