Effects of sodium salicylate on epithelial cells of the rectal mucosa of the rat: a light and electron microscopic study.
Effects of sodium salicylate on epithelial cells of the rectal mucosa of the rat: a light and electron microscopic study.
复制标题
水杨酸钠对大鼠直肠粘膜上皮细胞的影响：光学和电子显微镜研究。
DOI：
10.1016/0024-3205(83)90656-2
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发表时间：
1983
期刊：
影响因子：
6.1
通讯作者：
Caldwell,L
中科院分区：
文献类型：
--
作者：
Sithigorngul,P;Burton,P;Nishihata,T;Caldwell,L
使用结扎法，将大鼠直肠上皮暴露于2%水杨酸钠，并使用光镜和电子显微镜方法测定：1）上皮对标记染料台盼蓝的渗透性，和2）以上皮表面破坏表示的损伤。直肠粘膜暴露于水杨酸在pH 4.8，7.0和9.0，根皮苷预处理的影响进行了研究。结果表明，2%水杨酸钠在pH 7.0时对直肠上皮细胞的损伤非常小，同时增强了它们对台盼蓝的渗透性，这种作用在冲洗掉水杨酸钠后被逆转。水杨酸引起的主要细胞变化是上皮细胞微绒毛上糖萼丝的长度或分布减少。还注意到根皮苷预处理抵消了水杨酸盐处理的一些影响。
Using a ligation method, rat rectal epithelium was exposed to 2% sodium salicylate, and light and electron microscopic methods were used to assay for: 1) permeability of the epithelium to a marker dye, trypan blue, and 2) damage expressed in terms of disruption of the epithelial surface. Rectal mucosa was exposed to salicylate at pH 4.8, 7.0, and 9.0, and the effects of pretreatment with phlorizin were also studied. Results indicated that 2% sodium salicylate does very little damage to rectal epithelial cells at pH 7.0 while enhancing their permiability to trypan blue, an effect that is reversed upon washing out the sodium salicylate. The major cellular change induced by salicylate was a reduction in the length or distribution of glycocalyx filaments on microvilli of epithelial cells. It was also noted that pretreatment with phlorizin counteracted some of the effects of salicylate treatment.