Complete mitochondrial DNA sequence of Conger myriaster (Teleostei: Anguilliformes):: Novel gene order for vertebrate mitochondrial genomes and the phylogenetic implications for anguilliform families
Complete mitochondrial DNA sequence of Conger myriaster (Teleostei: Anguilliformes):: Novel gene order for vertebrate mitochondrial genomes and the phylogenetic implications for anguilliform families
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DOI：
10.1007/s002390010161
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发表时间：
2001-04-01
影响因子：
3.9
通讯作者：
Nishida, M
中科院分区：
文献类型：
--
作者：
Inoue, JG;Miya, M;Nishida, M
测定了海鳗线粒体基因组的全序列。海鳗（Elopomorpha：Anguilliformes），使用基于PCR的方法，采用长PCR技术和许多鱼类通用引物。虽然基因组[18.705个碱基对（bp）]包含与其他脊椎动物相同的37个线粒体基因[2个核糖体RNA（rRNA），22个转运RNA（tRNA）和13个蛋白质编码基因]，但基因顺序与其他脊椎动物的记录不同。在典型的脊椎动物中，ND 6、tRNA（Glu）和tRNA（Pro）基因位于ND 5基因和控制区之间，而在C. myriaster的基因已经被转移到控制区和tRNA（Phe）基因之间的位置，所述控制区和tRNA（Phe）基因在典型的脊椎动物中连续地位于12 S rRNA基因的5'末端。这一基因序列与最近报道的四个鸟类谱系的基因序列相似，后者在ND 5基因和控制区之间缺少ND 6、tRNA（Glu）和tRNA（Pro）基因，但在C.这可能是由于在两个亲缘关系较远的生物群体中，基因区域的串联重复和随后的基因缺失模式不同。对来自11个科的11种鳗类动物和1个外类群的鳗类动物的NDS-cyt B区序列进行分析，发现与C. myriaster为另外4科所共有，隶属于Congroidei亚目。虽然新的基因顺序的4个谱系的鸟类被认为有多个独立的起源，系统发育分析使用的线粒体12 S rRNA和cyt B基因的核苷酸序列表明，新的基因顺序的5个鳗科起源于一个单一的祖先物种。
The complete nucleotide sequence of the mitochondrial genome was determined for a conger eel. Conger myriaster (Elopomorpha: Anguilliformes), using a PCR-based approach that employs a long PCR technique and many fish-versatile primers. Although the genome [18.705 base pairs (bp)] contained the same set of 37 mitochondrial genes [two ribosomal RNA (rRNA), 22 transfer RNA (tRNA), and 13 protein-coding genes] as found in other vertebrates, the gene order differed from that recorded for any other vertebrates. In typical vertebrates, the ND6, tRNA(Glu), and tRNA(Pro) genes are located between the ND5 gene and the control region, whereas the former three genes, in C. myriaster, have been translocated to a position between the control region and the tRNA(Phe) gene that are contiguously located at the 5' end of the 12S rRNA gene in typical vertebrates. This gene order is similar to the recently reported gene order in four lineages of birds in that the latter lack the ND6, tRNA(Glu), and tRNA(Pro) genes between the ND5 gene and the control region, however, the relative position of the tRNA(Pro) to the ND6-tRNA(Glu) genes in C. myriaster was different from that in the four birds, which presumably resulted from different patterns of tandem duplication of gene regions followed by gene deletions in two distantly related groups of organisms. Sequencing of the NDS-cyt b region in 11 other anguilliform species, representing 11 families, plus one outgroup species, revealed that the same gene order as C. myriaster was shared by another 4 families, belonging to the suborder Congroidei. Although the novel gene orders of four lineages of birds were indicated to have multiple independent origins, phylogenetic analyses using nucleotide sequences from the mitochondrial 12S rRNA and cyt b genes suggested that the novel gene orders of the five anguilliform families had originated in a single ancestral species.