TALEN-mediated mutagenesis in zebrafish reveals a role for r-spondin 2 in fin ray and vertebral development
TALEN-mediated mutagenesis in zebrafish reveals a role for r-spondin 2 in fin ray and vertebral development
复制标题
TALEN 介导的斑马鱼诱变揭示了 r-spondin 2 在鳍条和脊椎发育中的作用
DOI：
10.1016/j.febslet.2014.10.015
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发表时间：
2014
期刊：
影响因子：
3.5
通讯作者：
H.
中科院分区：
文献类型：
--
作者：
Tatsumi;Y.;Takeda;M.;Matsuda;M.;Suzuki;T.;Yokoi;H.
R-spondin（Rspo）编码调节Wnt信号通路的多结构域蛋白。两个distinctrspo 2斑马鱼突变体产生的TALEN介导的诱变：无效突变体，rspo 2null，缺乏所有的功能结构域，和亚纯型突变体，rspo 2 tsp，缺乏两个N-末端结构域。突变体主要分析骨骼系统的异常。在therspo 2 tsp突变体中，鳍条骨架正常形成，但在therspo 2nullmutants中没有。在两种突变体中均观察到神经/血管弓和肋骨发育不全。因此，tworspo 2突变体有助于确定Rspo 2在骨骼发育中的功能，以及多个Rspo 2结构域之间的功能差异。
R-spondin(Rspo) encodes a multi-domain protein that modulates the Wnt-signaling pathway. Two distinctrspo2zebrafish mutants were generated by TALEN-mediated mutagenesis: a null mutant,rspo2null, lacking all functional domains, and a hypomorphic mutant,rspo2tsp, lacking the two N-terminal domains. Mutants were analyzed mainly for abnormalities in the skeletal system. Fin ray skeletons were formed normally in therspo2tspmutants, but were absent from therspo2nullmutants. Hypoplasia of the neural/hemal arches and ribs was observed in both mutants. Thus, the tworspo2mutants help to identify the functions of Rspo2 in skeletogenesis, as well as functional differences among multiple Rspo2 domains.