Ratiometric Bioluminescent Indicator for Simple and Rapid Diagnosis of Bilirubin
Ratiometric Bioluminescent Indicator for Simple and Rapid Diagnosis of Bilirubin
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DOI：
10.1021/acssensors.0c02000
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发表时间：
2021-01-14
期刊：
影响因子：
8.9
通讯作者：
Nagai, Takeharu
中科院分区：
文献类型：
--
作者：
Itoh, Yukino;Hattori, Mitsuru;Nagai, Takeharu
人体血液中的胆红素作为一个人的身体状况的一般指标是非常重要的，因为它的浓度在几种疾病的影响下发生变化。特别是在新生儿中，黄疸是最常见的涉及非结合胆红素（UCBR）的疾病之一，会引起核黄疸和耳聋等严重症状。因此，经常测量血液中的UCBR水平是很重要的。在这里，我们报告的比率生物发光指示剂，BABI（胆红素评估与生物发光指示剂），改变发射颜色从蓝色到绿色取决于样品中的UCBR浓度。由于使用了比吸收和荧光信号具有更高信噪比的生物发光信号，BABI能够使用智能手机相机对小血样进行高灵敏度和定量的UCBR检测。使用BABI建立UCBR测量测定法提供了使用生物发光指示剂和多功能移动终端进行基于血液的诊断的简单快速方法的可能性。
Bilirubin in human blood is highly important as a general index of one's physical condition because its concentration changes under the influence of several diseases. In particular, in newborns, jaundice is one of the most common diseases involving unconjugated bilirubin (UCBR), causing serious symptoms such as nuclear jaundice and deafness. Therefore, a frequent measurement of the UCBR levels in the blood is important. Here, we report a ratiometric bioluminescent indicator, BABI (bilirubin assessment with a bioluminescent indicator), that changes the emission color from blue to green depending on the UCBR concentration in a sample. Owing to the use of a bioluminescence signal that has a higher signal-to-noise ratio than the absorption and fluorescence signal, BABI enables highly sensitive and quantitative detection of UCBR for small blood samples using a smartphone camera. The establishment of a UCBR measurement assay using BABI provides the possibility of a simple and rapid method for blood-based diagnosis using bioluminescent indicators and a versatile mobile device.