Static and Dynamic Hypergravity Responses of Osteoblasts and Osteoclasts in Medaka Scales
Static and Dynamic Hypergravity Responses of Osteoblasts and Osteoclasts in Medaka Scales
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DOI：
10.2108/zsj.30.217
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发表时间：
2013-03
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通讯作者：
S. Yano;K. Kitamura;Y. Satoh;Masaki Nakano;A. Hattori;T. Sekiguchi;M. Ikegame;H. Nakashima;Katsunori Omori;K. Hayakawa;A. Chiba;Y. Sasayama;S. Ejiri;Y. Mikuni‐Takagaki;H. Mishima;H. Funahashi;T. Sakamoto;N. Suzuki
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作者：
S. Yano;K. Kitamura;Y. Satoh;Masaki Nakano;A. Hattori;T. Sekiguchi;M. Ikegame;H. Nakashima;Katsunori Omori;K. Hayakawa;A. Chiba;Y. Sasayama;S. Ejiri;Y. Mikuni‐Takagaki;H. Mishima;H. Funahashi;T. Sakamoto;N. Suzuki
Fish scales are a form of calcified tissue similar to that found in human bone. In medaka scales, we detected both osteoblasts and osteoclasts and subsequently developed a new scale assay system. Using this system, we analyzed the osteoblastic and osteoclastic responses under 2-, 3-, and 4-gravity (G) loading by both centrifugation and vibration. After loading for 10 min, the scales from centrifugal and vibration loading were incubated for 6 and 24 hrs, respectively, after which the osteoblastic and osteoclastic activities were measured. Osteoblastic activity significantly increased under 2- to 4-G loading by both centrifugation and vibration. In contrast, we found that osteoclastic activity significantly decreased under 2- and 3-G loading in response to both centrifugation and vibration. Under 4-G loading, osteoclastic activity also decreased on centrifugation, but significantly increased under 4-G loading by vibration, concomitant with markedly increased osteoblastic activity. Expression of the receptor activator of the NF-B ligand (RANKL), an activation factor of osteoclasts expressed in osteoblasts, increased significantly under 4-G loading by vibration but was unchanged by centrifugal loading. A protein sequence similar to osteoprotegerin (OPG), which is known as an osteoclastogenesis inhibitory factor, was found in medaka using our sequence analysis. The ratio of RANKL/OPG-like mRNAs in the vibration-loaded scales was significantly higher than that in the control scales, although there was no difference between centrifugal loaded scales and the control scales. Accordingly, medaka scales provide a useful model by which to analyze bone metabolism in response to physical strain.
