Translocation of σ70 with RNA polymerase during transcription:: Fluorescence resonance energy transfer assay for movement relative to DNA
Translocation of σ70 with RNA polymerase during transcription:: Fluorescence resonance energy transfer assay for movement relative to DNA
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DOI：
10.1016/s0092-8674(01)00464-0
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发表时间：
2001-08-24
期刊：
影响因子：
64.5
通讯作者：
Ebright, RH
中科院分区：
文献类型：
--
作者：
Mukhopadhyay, J;Kapanidis, AN;Ebright, RH
利用荧光共振能量转移，我们发现，在大多数转录复合物中，sigma（70）在起始到延伸的转变过程中并没有从RNA聚合酶中释放出来，相反，它仍然与RNA聚合酶相关，并与RNA聚合酶一起移位。结果反驳了在起始和延伸中存在必要的亚单位组成差异和相应的必要机制差异的假设。本报告的方法应推广到监测任何分子相对于任何核酸的运动。
Using fluorescence resonance energy transfer, we show that, in the majority of transcription complexes, sigma (70) is not released from RNA polymerase upon transition from initiation to elongation, but, instead, remains associated with RNA polymerase and translocates with RNA polymerase. The results argue against the presumption that there are necessary subunit-composition differences, and corresponding necessary mechanistic differences, in initiation and elongation. The methods of this report should be generalizable to monitor movement of any molecule relative to any nucleic acid.