Laparoscopic left hepatectomy in swine: a safe and feasible technique.

Laparoscopic left hepatectomy in swine: a safe and feasible technique.
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DOI:
10.4142/jvs.2014.15.3.417
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发表时间:
2014
影响因子:
1.8
通讯作者:
Wang HB
Wang HB
中科院分区:
农林科学4区
文献类型:
--
作者:
Zhang H;Liu T;Wang Y;Liu HF;Zhang JT;Wu YS;Lei L;Wang HB

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为猪左肝切除建立了一种纯腹腔镜四孔入路。左侧两肝叶放置聚乙烯环,用于牵引和提举。接下来,使用双排真丝缝线对肺叶进行穿透性结扎,以控制出血。使用单极钩电极完成直接肝横切,无需仔细解剖肝左静脉。用纤维蛋白胶凝结和封闭肝脏的原始表面。通过扩大的门静脉取回肺叶。所有猪均在无特殊器械的情况下完成了腹腔镜肝叶切除术,平均手术时间为179±9min。未观察到明显的围手术期并发症。平均每叶切除重量为180±51g,术后2周左右全血细胞计数、血清有机物和酶水平恢复正常。在尸检过程中,观察到肝脏原始表面与胃壁和大网膜的粘连。没有发现其他异常。这种微创猪左肝切除技术可以作为研究肝脏疾病和再生的有用模型,并为改进肝胆外科手术提供临床前信息。
A purely laparoscopic four-port approach was created for left hepatectomy in pigs. A polyethylene loop was placed on the left two hepatic lobes for traction and lift. Next, penetrating ligation of the lobes using of a double row of silk sutures was performed to control bleeding. A direct hepatic transection was completed using a monopolar hook electrode without meticulous dissection of the left hepatic vein. The raw surface of the liver was coagulated and sealed with fibrin glue. Lobes were retrieved through an enlarged portal. Laparoscopic hepatic lobectomy was completed in all pigs without the use of specialized instruments and with a mean operative time of 179 ± 9 min. No significant perioperative complications were observed. The average weight of each resected lobe was 180 ± 51 g. Complete blood count as well as serum organics and enzyme levels normalized after about 2 weeks. During necropsy, adhesion of the hepatic raw surface to the gastric wall and omentum were observed. No other abnormalities were identified. This minimally invasive left hepatectomy technique in swine could serve as a useful model for investigating liver diseases and regeneration, and offer preclinical information to improve hepatobiliary surgical procedures.
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