Esophageal replacement in rat using porcine intestinal submucosa as a patch or a tube-shaped graft
Esophageal replacement in rat using porcine intestinal submucosa as a patch or a tube-shaped graft
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DOI：
10.1111/j.1442-2050.2006.00574.x
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发表时间：
2006-01-01
影响因子：
2.6
通讯作者：
Patricio, J.
中科院分区：
文献类型：
--
作者：
Lopes, M. F.;Cabrita, A.;Patricio, J.
本研究采用一种新的食道再生模型，比较了猪肠黏膜下层(SIS)补片移植物和SIS管状移植物在食道置换中的效果。对两个SIS治疗组的临床功能以及肉眼和显微形态进行评估。我们在大鼠身上进行了食道半周切除，然后使用SIS补片移植物修复缺损(组I)或节段性食道切除后插入SIS管移植物(组II)。两组间28天存活率差异有统计学意义(I组为100%，II组为0%)。与II组大鼠不同，II组大鼠在术后第一个月内因食道功能障碍而死亡，而I组所有存活的动物在术后几天内恢复了正常的固体饮食，没有食道功能障碍的迹象，体重也增加了。吞钡检查未发现瘘管、显著狭窄或憩室的证据。未发现血液学或血清生化异常。到第150天，SIS贴片被食道来源的组织取代。在大鼠模型中，使用SIS的补片移植技术似乎可以诱导食道再生，并提供最初和长期满意的功能，而使用SIS的管状移植技术则不成功。
This study compares the efficacy of porcine intestinal submucosa (SIS) patch graft versus SIS-tube graft in esophageal replacement, using a novel esophageal regeneration model. Clinical function, as well as macroscopic and microscopic morphology were evaluated in both SIS-treated groups. We performed semi-circumferential esophageal excision followed by repair of the defect using either a SIS-patch graft (group I) or segmental esophageal excision followed by a SIS-tube interposition graft (group II) in rats. The 28-day survival rate was significantly different between the SIS-treated groups (100% in group I vs. 0% in group II). Unlike the rats in group II, which died within the first postoperative month due to esophageal dysfunction, all surviving animals in group I resumed a normal solid diet within a few days after surgery, without signs of esophageal dysfunction and gained weight. Barium swallow studies showed no evidence of fistula, significant stenosis or diverticula. No hematological or serum biochemistry abnormalities were found. By day 150 the SIS patch was replaced by esophageal-derived tissues. In the rat model, a patch graft technique using SIS appeared to induce esophageal regrowth and provided an initial and long-term satisfactory function, while a tube-shaped graft technique using SIS was unsuccessful.