Isolation of the gene for a glycophorin-binding protein implicated in erythrocyte invasion by a malaria parasite.
Isolation of the gene for a glycophorin-binding protein implicated in erythrocyte invasion by a malaria parasite.
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分离与疟原虫入侵红细胞有关的血型糖蛋白结合蛋白基因。
DOI：
10.1126/science.3883491
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Perkins,M
中科院分区：
文献类型：
--
作者：
Ravetch,JV;Kochan,J;Perkins,M
恶性疟原虫是感染人类的最致命的疟疾寄生虫，在其脊椎动物宿主中经历了三个周期的发育，并引发阶段特异性免疫反应。免疫反应的这一阶段特异性使得很难分离出对开发疟疾疫苗有用的抗原。分离和鉴定了恶性疟原虫血清白蛋白结合蛋白的互补DNA克隆。在寄生虫入侵宿主细胞的过程中，这种蛋白质与红细胞受体血糖素相互作用。在大肠杆菌中表达的这种蛋白的抗原决定簇已被用来产生一种血糖素结合蛋白的抗体。这些抗体显示裂殖体特异性免疫荧光，并与裂殖子蛋白发生反应。这些决定因素的初级序列揭示了一个150个核苷酸的串联重复序列，编码一个50个氨基酸的重复。恶性疟原虫血糖蛋白结合蛋白的特征代表了设计血清学试剂以阻止寄生虫在脊椎动物宿主中发展的一种方法。
Plasmodium falciparum, the most lethal of the malarial parasites that infect humans, undergoes three cycles of development in its vertebrate host and elicits stage-specific immune responses. This stage specificity of the immune response has made it difficult to isolate antigens that would be useful in developing a vaccine against malaria. A complementary DNA clone for a glycophorin-binding protein ofPlasmodium falciparummerozoites has been isolated and characterized. The protein interacts with glycophorin, the erythrocyte receptor, during invasion of the host cell by the parasite. Antigenic determinants of this protein expressed in Escherichia coli have been used to produce antibodies to a glycophorin-binding protein. The antibodies show schizont-specific immunofluorescence and react with the merozoite protein. The primary sequence of these determinants reveals a 150-nucleotide tandem-repeating sequence coding for a 50-amino-acid repeat. The characterization of thePlasmodium falciparumglycophorin-binding protein represents one approach toward designing serologic agents to block the parasite's development in the vertebrate host.