Messenger RNA for manganese and copper-zinc superoxide dismutases in hepatomas: correlation with degree of differentiation.
Messenger RNA for manganese and copper-zinc superoxide dismutases in hepatomas: correlation with degree of differentiation.
复制标题
肝癌中锰和铜锌超氧化物歧化酶的信使 RNA：与分化程度的相关性。
DOI：
10.1016/s0006-291x(89)80006-3
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发表时间：
1989
影响因子：
3.1
通讯作者：
DeLeo,ME
中科院分区：
文献类型：
--
作者：
Galeotti,T;Wohlrab,H;Borrello,S;DeLeo,ME
总RNA和聚腺苷酸化RNA已从两种不同分化程度的Morris肝癌和相应荷瘤近交大鼠的正常肝脏中分离出来。 mRNA分析通过Northern杂交进行，使用32P-dA尾合成脱氧寡核苷酸探针，Mn超氧化物歧化酶(SOD)为33聚体，CuZnSOD为36聚体，源自大鼠酶cDNA的核苷酸序列。使用 MnSOD 探针已鉴定出两种不同的 mRNA 种类（约 850 和 1080 个核苷酸）。 CuZnSOD 由约 720 个核苷酸的单个信息翻译而来。肝癌9618A（高分化）和3924A（低分化）中MnSOD mRNA总浓度分别降低43%和57%。 CuZnSOD mRNA 在肝癌 9618A 中几乎没有变化，而在肝癌 3924A 中减少了 80%。酶活性和 mRNA 水平的比较表明仅肝癌 3924A 具有良好的相关性，表明两种 SOD 的变化均在翻译前受到调节。从获得的数据还推断，MnSOD 的 mRNA 水平比 CuZnSOD 的 mRNA 水平更容易对分化的变化做出反应。
Total and polyadenylylated RNA have been isolated from two Morris hepatomas with different degree of differentiation and from the normal liver of the corresponding tumor-bearing inbred rats. The analysis of mRNA has been performed by Northern hybridization using32P-dA-tailed synthetic deoxyoligonucleotide probes, 33-mer for Mn superoxide dismutase (SOD) and 36-mer for CuZnSOD, derived from the nucleotide sequences of the rat enzyme cDNAs. Two distinct mRNA species (about 850 and 1080 nucleotides) have been identified by using the MnSOD probe. CuZnSOD is translated from a single message of about 720 nucleotides. The total MnSOD mRNA concentration is decreased by 43% and 57% in the hepatomas 9618A (highly differentiated) and 3924A (poorly differentiated), respectively. CuZnSOD mRNA is practically unchanged in the hepatoma 9618A whereas it is reduced by 80% in the hepatoma 3924A. Comparison of the enzyme activities and mRNA levels indicates a good correlation only for hepatoma 3924A, suggesting that the changes of both SODs are regulated pretranslationally. From the data obtained it is also inferred that the mRNA levels of MnSOD respond more readily than those of CuZnSOD to changes in differentiation.