Highly efficient detection of salbutamol in environmental water samples by an enzyme immunoassay
Highly efficient detection of salbutamol in environmental water samples by an enzyme immunoassay
复制标题
酶联免疫分析法高效检测环境水样中的沙丁胺醇
DOI：
10.1016/j.scitotenv.2017.08.324
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发表时间：
2018-02-01
影响因子：
9.8
通讯作者：
Luan, Jiawen
中科院分区：
文献类型：
--
作者：
Liu, Zhenjiang;Zhang, Bo;Luan, Jiawen
Salbutamol (SAL) has potential hazardous effects on health, and has been found in natural water globally. To protect consumers from risks related to SAL residues, a fast and highly sensitive enzyme-linked immunosorbent assay (ELISA) was developed for the detection of SAL in environmental water samples. SAL hapten was synthesized and conjugated to proteins to be used as the immunogen for producing polyclonal antibodies. Under optimized conditions, the ELISA showed a 50% inhibitory concentration (IC50 value) of 0.466 mu g/L, with a limit of detection (LOD, IC10 value) of 0.021 mu g/L. The LOD of the ELISA was 5-fold lower than the maximum residue limits. The average recoveries of SAL fromenvironmental samples, including tapwater, riverwater and wastewater, ranged from 80.1 to 115.6%, which iswell within the requirements of residue detection. Moreover, the results of the ELISA correlated wellwith high-performance liquid chromatography results, with a correlation coefficient of 0.988. These results suggest that the proposed ELISA method has potential application for the monitoring of SAL residues in environmental water samples. (C) 2017 Elsevier B.V. All rights reserved.
