Free-flow electrophoresis with electrode-less injection molded chips
Free-flow electrophoresis with electrode-less injection molded chips
复制标题
使用无电极注塑芯片进行自由流动电泳
DOI：
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发表时间：
2011
期刊：
影响因子：
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通讯作者：
D. Belder
中科院分区：
文献类型：
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作者：
Stefan Köhler;H. Becker;V. Beushausen;W. Hüttner;H. Wackerbarth;E. Beckert;S. Howitz;D. Belder
在这项工作中，我们提出了第一种利用注射成型作为微加工工艺的低成本自由流动电泳（FFE）设备的方法，该方法有可能以使其在商业上可行的成本制造FFE芯片。这是通过实现一种新的直接的微自由流动电泳（μFFE）设计来实现的，通过实现小型化的分区棒，确保无气泡电泳分离和有效的电气连接。这在分离区和电极通道之间产生了一个高度为20 μm，宽度为500 μm的开放间隙。热塑性μFFE芯片已经准备好使用，不需要随后的劳动密集型膜或盐桥的实施来将电极通道与分离区分开。
In this work we present the first approach towards low-cost free-flow electrophoresis (FFE) devices utilizing injection molding as a microfabrication process which has the potential to manufacture FFE chips at a cost which make their use commercially viable. This is achieved by realizing a new straightforward micro free-flow electrophoresis (μFFE) design ensuring both, bubble free electrophoretic separation and effective electrical connection by implementing miniaturized partitioning bars. This creates a defined open gap of 20 μm in height and 500 μm in width between separation zone and electrode channels. The thermoplastic μFFE chips are ready to use, there is no need for a subsequent labor-intensive implementation of membranes or salt bridges to separate the electrode channels from the separation zone.
影响因子：
7.4
作者：
Song, Yong-Ak;Chan, Michael;Celio, Chris;Tannenbaum, Steven R.;Wishnok, John S.;Han, Jongyoon
通讯作者：
Han, Jongyoon