Nanocomposite liposomes containing quantum dots and anticancer drugs for bioimaging and therapeutic delivery: a comparison of cationic, PEGylated and deformable liposomes
Nanocomposite liposomes containing quantum dots and anticancer drugs for bioimaging and therapeutic delivery: a comparison of cationic, PEGylated and deformable liposomes
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DOI：
10.1088/0957-4484/24/32/325101
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发表时间：
2013-08
期刊：
影响因子：
3.5
通讯作者：
C. Wen;C. Sung;I. Aljuffali;Yu-jie Huang;Jia-You Fang
中科院分区：
文献类型：
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作者：
C. Wen;C. Sung;I. Aljuffali;Yu-jie Huang;Jia-You Fang
制备了装载量子点和抗癌药物的多功能脂质体，用于同时进行生物成像和给药。不同的配方，包括阳离子，聚乙二醇化和可变形脂质体，比较其治疗效果。我们对这些脂质体的理化性质进行了评价。利用细胞毒性、细胞迁移、细胞摄取、体内黑色素瘤成像和肿瘤内药物积累等实验平台对所制备的脂质体进行了检测。各种纳米复合脂质体的平均粒径为92 ~ 134 nm。透射电镜证实在脂质体双层中存在量子点。聚乙二醇（PEG）和Span 20在脂质体中的掺入大大增加了双层的流动性。脂质体提供喜树碱和伊立替康的缓释。细胞毒性和细胞迁移实验表明，阳离子脂质体的活性优于其他载体。阳离子脂质体内化后在黑色素瘤细胞中也显示出明显的荧光信号。脂质体在瘤内给药于患有黑色素瘤的小鼠。阳离子脂质体在肿瘤中荧光最亮，其次是经典脂质体。对于阳离子纳米系统，这个信号可以持续24小时。自由对照的喜树碱瘤内蓄积为35 nmol g−1；负载阳离子脂质体后可增加到50 nmol g−1。然而，将伊立替康包封在脂质体中并没有进一步增加肿瘤内的药物积累。阳离子脂质体作为纳米载体在生物成像和治疗方面都优于其他脂质体。
Multifunctional liposomes loaded with quantum dots (QDs) and anticancer drugs were prepared for simultaneous bioimaging and drug delivery. Different formulations, including cationic, PEGylated and deformable liposomes, were compared for their theranostic efficiency. We had evaluated the physicochemical characteristics of these liposomes. The developed liposomes were examined using experimental platforms of cytotoxicity, cell migration, cellular uptake, in vivo melanoma imaging and drug accumulation in tumors. The average size of various nanocomposite liposomes was found to be 92–134 nm. Transmission electron microscopy confirmed the presence of QDs within liposomal bilayers. The incorporation of polyethylene glycol (PEG) and Span 20 into the liposomes greatly increased the fluidity of the bilayers. The liposomes provided sustained release of camptothecin and irinotecan. The cytotoxicity and cell migration assay demonstrated superior activity of cationic liposomes compared with other carriers. Cationic liposomes also showed a significant fluorescence signal in melanoma cells after internalization. The liposomes were intratumorally administered to a melanoma-bearing mouse. Cationic liposomes showed the brightest fluorescence in tumors, followed by classical liposomes. This signal could last for up to 24 h for cationic nanosystems. Intratumoral accumulation of camptothecin from free control was 35 nmol g−1; it could be increased to 50 nmol g−1 after loading with cationic liposomes. However, encapsulation of irinotecan into liposomes did not further increase intratumoral drug accumulation. Cationic liposomes were preferable to other liposomes as nanocarriers in both bioimaging and therapeutic approaches.