High expression of octamer transcription factor 1 in cervical cancer.
High expression of octamer transcription factor 1 in cervical cancer.
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八聚体转录因子1在宫颈癌中高表达
DOI：
10.3892/ol.2014.2023
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发表时间：
2014-06
期刊：
影响因子：
2.9
通讯作者：
Xue M
中科院分区：
文献类型：
--
作者：
Xiao S;Liao S;Zhou Y;Jiang B;Li Y;Xue M
宫颈癌是全世界女性中第二常见的恶性肿瘤。宫颈癌发生的关键病因包括乳头瘤病毒感染、癌基因和抑癌基因的结构或功能突变。八聚体转录因子1（OCT1）的异常变化与肿瘤进展和患者较差的生存率相关。然而，关于 OCT1 在宫颈癌中的作用却知之甚少。在本研究中，通过流式细胞术、蛋白质印迹分析和定量聚合酶链反应（qPCR）来鉴定宫颈癌组织和癌旁非癌组织中差异表达的OCT1。宫颈癌中归一化的OCT1基因表达量是癌旁组织的5.98倍。蛋白质印迹分析和流式细胞术评估了 OCT1 蛋白的水平。两种差异技术的结果表明，OCT1蛋白在宫颈癌组织中表达量较高，这与qPCR结果相符。最后，由于OCT1是microRNA（miR）-1467、-1185、-4493和-3919的潜在靶基因，因此分析了它们在宫颈癌组织和癌旁非癌组织中的表达水平；它们在宫颈癌样本中下调了约 45%。本研究结果表明OCT1在宫颈癌组织中高表达，提示OCT-1在宫颈癌中可能具有重要意义。
Cervical carcinoma is the second most prevalent malignancy in females worldwide. The crucial etiologic factors involved in the development of cervical carcinoma include infection with papillomavirus, and the structural or functional mutation of oncogenes and tumor suppressor genes. The abnormal change of octamer transcription factor 1 (OCT1) is associated with tumor progression and a poor patient survival rate. However, little is known regarding the effect of OCT1 in cervical cancer. In the present study, flow cytometry, western blot analysis and quantitative polymerase chain reaction (qPCR) were peformed to identify differentially expressed OCT1 in cervical cancer tissue and adjacent non-cancerous tissues. The normalized OCT1 gene expression in cervical cancer was 5.98 times higher compared with the adjacent non-cancerous tissues. Western blot analysis and flow cytometry assessed the levels of OCT1 protein. The results of these two differential techniques showed that the protein expression level of OCT1 was greater in cervical cancer tissues, which corresponded with the qPCR results. Finally, as OCT1 is a potential target gene for microRNA (miR)-1467, -1185, -4493 and -3919, their expression levels were analyzed in cervical cancer tissues and adjacent non-cancerous tissues; they were downregulated by ~45% in the cervical cancer samples. The results of the present study showed that OCT1 is highly expressed in cervical cancer tissues and indicated that OCT-1 may be significant in cervical cancer.
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