Technique for High-Quality Protein Crystal Growth by Control of Subgrain Formation under an External Electric Field
Technique for High-Quality Protein Crystal Growth by Control of Subgrain Formation under an External Electric Field
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DOI：
10.3390/cryst6080095
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发表时间：
2016-08
期刊：
影响因子：
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通讯作者：
H. Koizumi;S. Uda;K. Fujiwara;M. Tachibana;K. Kojima;J. Nozawa
中科院分区：
文献类型：
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作者：
H. Koizumi;S. Uda;K. Fujiwara;M. Tachibana;K. Kojima;J. Nozawa
采用x射线衍射（XRD）测定了外加电场和不外加电场作用下生长的四方蛋清（HEW）溶菌酶晶体的x射线衍射（XRD）岩石曲线，并根据各岩石曲线剖面的半最大值全宽度（FWHMs）来评价晶体质量。在1 MHz外电场下生长的四边形HEW溶菌酶晶体的平均fwhm小于无外电场下生长的晶体，特别是在12 - 120 - 0反射时。在1 MHz的外加电场作用下，四边形HEW溶菌酶晶体的均匀性也得到了改善。讨论了在外加电场下生长的四边形HEW溶菌酶晶体质量的改善，重点讨论了亚颗粒的形成。此外，还讨论了蛋白质晶体中杂质的掺入对亚粒取向错误的影响。
X-ray diffraction (XRD) rocking-curves were measured for tetragonal hen egg white (HEW) lysozyme crystals grown with and without application of an external electric field, and the crystal quality was assessed according to the full width at half-maximums (FWHMs) of each rocking-curve profile. The average FWHMs for tetragonal HEW lysozyme crystals grown with an external electric field at 1 MHz were smaller than those for crystals grown without, especially for the 12 12 0 reflection. The crystal homogeneity of the tetragonal HEW lysozyme crystals was also improved under application of an external electric field at 1 MHz, compared to that without. Improvement of the crystal quality of tetragonal HEW lysozyme crystals grown under an applied field is discussed with a focus on subgrain formation. In addition, the origin of subgrain misorientation is also discussed with respect to the incorporation of impurities into protein crystals.