RETINAL VASCULAR ENDOTHELIUM EXPRESSES FIBRONECTIN AND CLASS-II HISTOCOMPATIBILITY COMPLEX ANTIGENS IN EXPERIMENTAL AUTOIMMUNE UVEITIS
RETINAL VASCULAR ENDOTHELIUM EXPRESSES FIBRONECTIN AND CLASS-II HISTOCOMPATIBILITY COMPLEX ANTIGENS IN EXPERIMENTAL AUTOIMMUNE UVEITIS
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DOI：
10.1016/0008-8749(87)90157-2
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发表时间：
1987-04-15
影响因子：
4.3
通讯作者：
NUSSENBLATT, RB
中科院分区：
文献类型：
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作者：
FUJIKAWA, LS;CHAN, CC;NUSSENBLATT, RB
为探讨视网膜血管内皮细胞在实验性自身免疫性葡萄膜炎（EAU）发生发展中的作用，我们研究了视网膜S抗原免疫的刘易斯大鼠视网膜血管中Ia抗原和FN的表达。对在疾病的各个阶段获得的冷冻组织进行免疫病理学研究。我们的研究结果表明，Ia抗原不存在于正常大鼠视网膜，有很少的FN存在于少数视网膜血管。在EAU的组织学和临床发病前1 ~ 2天，发现视网膜血管中FN增加。发现Ia抗原存在于视网膜血管中，与细胞浸润的最初迹象一致。在最大细胞浸润阶段，FN弥漫性存在于整个视网膜，以及在视网膜下腔，Ia抗原被发现弥漫性的细胞浸润。因此，FN和Ia抗原反映了EAU血管内皮细胞的免疫调节作用，可能在视网膜S抗原诱导的葡萄膜炎的发病机制中起重要作用。讨论了视网膜血管内皮细胞激活在葡萄膜炎视网膜炎症发展中的作用的两种可能机制。
To analyze the role of the retinal vascular endothelial cells in the development of experimental autoimmune uveitis (EAU), we studied the presence of Ia antigen and FN in retinal vessels of Lewis rats immunized with retinal S antigen. Immunopathologic studies were performed on frozen tissues obtained during various stages of the disease. Our results show that Ia antigen was not present in the normal rat retina, and there was very little FN present in a few retinal vessels. One to two days prior to the histologic and clinical onset of EAU, FN was found to be increased in the retinal vessels. Ia antigen was found to be present in the retinal vessels coincident with the first signs of cellular infiltration. During the stage of maximal cellular infiltration, FN was present diffusely throughout the retina, as well as in the subretinal space, and Ia antigen was found diffusely in the cellular infiltrate. Therefore, FN and Ia antigen reflect the immunomodulation of vascular endothelial cells in EAU, which may be very important in the pathogenesis of retinal S antigen-induced uveitis. Two possible mechanisms for the role of the activation of the retinal vascular endothelium in the development of retinal inflammation in uveitis are discussed.