Co-operative inhibition byfluoride and zinc of glucosyltransferase production and polysaccharicle synthesis by mutans streptococci in suspension cultures and biofilms
Co-operative inhibition byfluoride and zinc of glucosyltransferase production and polysaccharicle synthesis by mutans streptococci in suspension cultures and biofilms
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DOI：
10.1111/j.1574-6968.2005.00018.x
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发表时间：
2006-01-01
影响因子：
2.1
通讯作者：
Marquis, RE
中科院分区：
文献类型：
--
作者：
Koo, H;Sheng, JY;Marquis, RE
氟和锌，单独或组合在0.2 mM的浓度，抑制生产分泌的葡糖基转移酶的变形链球菌UA159生长在悬浮培养物。抑制不涉及生长抑制或饥饿。氟化物和锌也抑制葡聚糖生产，特别是不溶性葡聚糖，在补料分批生物膜。生物膜的抑制似乎与饥饿有关，如生物膜细胞中ATP池和嗜碘多糖水平显着降低所示。由于不溶性葡聚糖对变形链球菌的毒力非常重要，氟和锌的抑制作用可显著影响致龋性。
Fluoride and zinc, alone or in combination at concentrations of 0.2 mM, inhibited production-secretion of glucosyltranferases by Streptococcus mutans UA159 growing in suspension cultures. Inhibition did not involve growth inhibition or starvation. Fluoride and zinc also inhibited glucan production, especially insoluble glucan, in fed-batch biofilms. Inhibition of biofilms appeared to be associated with starvation as indicated by markedly decreased ATP pools and iodophilic polysaccharide levels in biofilm cells. As insoluble glucans are important for virulence of mutans streptococci, the inhibitory actions of fluoride and zinc could significantly affect cariogenicity.