Two-component dielectric dispersion impedance biosensor for in-line protein monitoring
Two-component dielectric dispersion impedance biosensor for in-line protein monitoring
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DOI：
10.1016/j.snb.2016.09.118
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发表时间：
2017-02-01
影响因子：
8.4
通讯作者：
Schmidt, B.
中科院分区：
文献类型：
--
作者：
Oseev, A.;Schmidt, M. -P.;Schmidt, B.
在目前的贡献中，我们展示了一种阻抗谱微流体传感器，它能够在与β和δ介电色散区相对应的宽频率范围内表征蛋白质溶液。所述传感器的主要应用是生物过程中的在线监测，其中对技术参数漂移的实时检测要求很高，以获得即时反馈影响。该传感器利用体积特性的检测，而无需应用固定涂层。它直接在流动中对蛋白质溶液的体积介电特性进行非破坏性和非侵入性分析。它主要分析涉及蛋白质复杂介电弛豫机制的宽频率范围内的介电谱，并恢复有关预定义蛋白质溶液的信息（如蛋白质浓度监测）。该传感器允许对蛋白质溶液进行连续的体积在线分析，而无需中断重新校准或纯化。然而，所提出的传感器的制造与几个技术挑战有关。在目前的贡献中，我们描述了传感器制造过程的技术，并报告了在磷酸盐缓冲盐水（PBS）中监测牛血清白蛋白（BSA）蛋白溶液的指示性实验结果。(C) 2016 Elsevier B.V.版权所有
In current contribution we demonstrate an impedance spectroscopy microfluidic sensor that is able to characterize protein solutions in a broad frequency range corresponding to beta and delta dielectric dispersion regions. The primary application of the described sensor is an in-line monitoring within bioprocesses where the real-time detection of drifts of technological parameters is highly demanded for the immediate feedback influence. The sensor utilizes the detection of volumetric properties without the application of immobilization coatings. It conducts non-destructive and non-invasive analysis of volumetric dielectric properties of protein solutions directly in a flow. It primarily analysis the dielectric spectrum in a broad frequency range where protein complex dielectric relaxation mechanisms are involved and restore information about the predefined protein solution (such as protein concentration monitoring). The sensor allows a continuous volumetric in-line analysis of protein solutions without interruption for re-calibration or purification. However, the manufacture of the proposed sensor is associated with several technological challenges. In current contribution we describe the technology of the sensor fabrication process and report the indicative experimental results of monitoring of bovine serum albumin (BSA) protein solution in phosphate-buffered saline (PBS). (C) 2016 Elsevier B.V. All rights reserved.