Structural analysis of vaccinia virus dls strain: Application as a new replication-deficient viral vector
Structural analysis of vaccinia virus dls strain: Application as a new replication-deficient viral vector
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DOI：
10.1006/viro.2002.1622
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发表时间：
2002-10-25
期刊：
影响因子：
3.7
通讯作者：
Miyamura, T
中科院分区：
文献类型：
--
作者：
Ishii, K;Ueda, Y;Miyamura, T
DIs是痘苗病毒DIE株的限制性宿主范围突变体，仅在鸡胚成纤维细胞中生长良好，但不能在大多数哺乳动物细胞中生长。在这项研究中，我们确定了一个主要的缺失（15.4 kbp），导致19个推定的开放阅读框架的损失在基因组的左端。我们构建了表达噬菌体T7聚合酶（T7 pol）基因的rDIs，并通过T7启动子下的报告荧光素酶基因表达，显示了其在多种哺乳动物细胞系中的表达。我们还表达了全长人类免疫缺陷病毒（HIV）-1 NL 432 gag基因。表达的gag基因产物在免疫小鼠中诱导高水平的细胞毒性T淋巴细胞。这些数据表明，DI作为一种有效的、瞬时的复制缺陷型病毒载体是有用的。(C)2002 Elsevier Science（美国）。
DIs is a restrictive host range mutant of vaccinia virus strain DIE that grows well only in chick embryo fibroblast cells but is unable to grow in most mammalian cells. In this study, we identified one major deletion (15.4 kbp) which results in the loss of 19 putative open reading frames in the left end of the genome. We then established a system to express foreign genes by inserting them into the deleted region of DIs. We constructed rDIs to express the bacteriophage T7 polymerase (T7pol) gene and showed the expression in various mammalian cell lines by reporter luciferase gene expression under the T7 promoter. We also expressed the full-length human immunodeficiency virus (HIV)-1 NL432 gag gene. The expressed gag gene product induced high levels of cytotoxic T lymphocytes in immunized mice. These data suggest that DIs is useful as an efficient, transient replication-deficient viral vector. (C) 2002 Elsevier Science (USA).