FETUS-SPECIFIC EXPRESSION OF A FORM OF CYTOCHROME-P-450 IN HUMAN LIVERS
FETUS-SPECIFIC EXPRESSION OF A FORM OF CYTOCHROME-P-450 IN HUMAN LIVERS
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DOI：
10.1021/bi00470a024
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发表时间：
1990-05-08
期刊：
影响因子：
2.9
通讯作者：
KAMATAKI, T
中科院分区：
文献类型：
--
作者：
KOMORI, M;NISHIO, K;KAMATAKI, T
研究了从胎儿和成人肝脏文库中分离的细胞色素P-450的发育调节表达形式，即由HFL33和NF25或HLP cDNAs编码的细胞色素P-450。以HFL33和NF25基因克隆的EcoRI片段为探针，分别与人胎肝和成人肝的RNA杂交。然而，当使用针对λHFL33编码区和3‘非编码区的寡核苷酸(分别为OLI-HFL和OLI-HFL3’‘)作为探针时，这些探针可与胎儿肝脏的RNA杂交，但不能与成人肝脏的RNA杂交。另一方面，针对NF25和HLP编码区的寡核苷酸探针(oli-nf)仅与成人肝脏的RNA呈阳性条带。这些结果表明，P-450(HFL33)在胎肝中特异表达，P-450NF和HLP在胎肝中均不表达，但其中一个或两者在成人肝中均有表达。
The developmentally regulated expression of forms of cytochrome P-450, namely, those encoded by .lambda.HFL33 and NF25 or HLp cDNAs, which were isolated from respective fetal and adult human liver cDNA libraries, was investigated. When EcoRI fragments of cDNA clones of .lambda.HFL33 and NF25 were used as probes, these probes hybridized with RNA from both fetal and adult human livers. However, when oligonucleotides specific to the coding and 3''-noncoding region of .lambda.HFL33 (oli-HFL and oli-HFL3'', respectively) were used as probes, these probes gave hybridizable bands with RNA from fetal but not adult livers. On the other hand, an oligonucleotide probe specific to the coding region of NF25 and HLp (oli-NF) gave positive bands with RNA only from adult livers. These results indicate that P-450(HFL33) is expressed specifically in fetal livers and that neither P-450NF nor HLp is expressed in fetal livers, but one or both are expressed in adult livers.