Effect of plaque accumulation and salivary factors on enamel demineralization and plaque composition in situ.
Effect of plaque accumulation and salivary factors on enamel demineralization and plaque composition in situ.
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DOI：
10.1590/s1517-74912003000400006
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发表时间：
2003-12
影响因子：
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通讯作者：
L. Tenuta;J. E. Lima;C. L. Cardoso;C. Tabchoury;J. Cury
中科院分区：
文献类型：
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作者：
L. Tenuta;J. E. Lima;C. L. Cardoso;C. Tabchoury;J. Cury
本研究评估了一些菌斑和唾液因素对原位龋进展的影响。对13名志愿者的唾液分泌率、缓冲能力和变形链球菌计数进行了测定。在三个不同的时间段，4，7和10天，他们每个人都戴着一个腭装置含有4个牛牙釉质块。他们在实验过程中使用了一种非氟化的水溶液，每天将20%的蔗糖溶液滴在块上10次。在每个时期后，在釉质块上形成的牙菌斑中定量变形链球菌（MS）、钙（Ca）和不溶性多糖（IP）。通过表面显微硬度评估牙釉质脱矿，并计算相对于基线值的表面显微硬度变化百分比（%SMC）。牙釉质脱矿发生在每一个菌斑积累期之后（p < 0.05），并且%SMC随时间增加（从13.8%增加到48.3%）。各实验时间点菌斑中Ca和IP浓度无统计学差异，但与%SMC之间存在显著相关性。无论是最初评估的唾液因素，还是斑块中的变形链球菌，都与%SMC无统计学显著相关性。结果表明，釉质脱矿是时间依赖性的，更相关的生物膜形成的组成比唾液因素的研究。
This study evaluated the effect of some plaque and salivary factors on caries progression in situ. The salivary secretion rate, buffering capacity and mutans streptococci counts from 13 volunteers were determined. For three distinct periods of time, 4, 7 and 10 days, each of them wore a palatal appliance containing 4 bovine enamel blocks. They used a non-fluoridated dentifrice during the experiment and a 20% sucrose solution was dripped onto the blocks 10 times a day. Mutans streptococci (MS), calcium (Ca), and insoluble polysaccharide (IP) were quantified in the dental plaque formed on the enamel blocks, after each period. Enamel demineralization was assessed by surface microhardness, and the percentage of surface microhardness change (%SMC) in relation to the baseline values was calculated. Enamel demineralization occurred after each period of plaque accumulation (p < 0.05), and the %SMC increased with time (from 13.8 to 48.3%). The concentrations of Ca and IP in plaque were not statistically different among the experimental times, but significant correlations were found between these concentrations and %SMC. Neither the salivary factors assessed initially nor mutans streptococci in plaque presented statistically significant correlations to %SMC. The results suggest that enamel demineralization is time-dependent and is more related to the composition of the biofilm formed than to the salivary factors studied.