Dual-signal amplified photoelectrochemical assay for DNA methyltransferase activity based on RGO-CdS: Mn nanoparticles and a CdTe@DNA network
Dual-signal amplified photoelectrochemical assay for DNA methyltransferase activity based on RGO-CdS: Mn nanoparticles and a CdTe@DNA network
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基于 RGO-CdS: Mn 纳米粒子和 CdTe@DNA 网络的 DNA 甲基转移酶活性双信号放大光电化学测定
DOI：
10.1016/j.snb.2019.127266
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Junsong Zheng
中科院分区：
文献类型：
--
作者：
Jing Luo;Lichao Fang;Huamin Liu;Quanjing Zhu;Hui Huang;Jun Deng;Feixue Liu;Yan Li;Junsong Zheng
DNA甲基转移酶（DNA MTase）已被证明能催化DNA甲基化，而DNA甲基化与癌症密切相关。因此，开发DNA MTase活性检测技术对癌症的早期诊断具有重要意义。本文以RGO-CdS:Mn纳米粒子和CdTe@DNA网络为光电材料，利用双信号放大系统制备了一种新型的超灵敏光电电化学（PEC）生物传感器，用于分析DNA MTase活性。首先在金电极上修饰RGO-CdS:Mn纳米颗粒作为衬底材料。然后，在电极上组装具有DNA甲基化识别序列的探针DNA和靶DNA。经过甲基化和消化的过程，甲基化的dsDNA可以。保留并进一步与CdTe@DNA网络杂交，从而产生更强的光电流。正如预期的那样，PEC响应与m.s sssi MTase浓度的对数成正比，在0.01 ~ 80 U/mL之间，检测限为0.0071 U/mL。该方法具有较好的MTase活性检测能力，可为肿瘤诊断提供新的思路和技术。
DNA methyltransferase (DNA MTase) has been shown to catalyze DNA methylation, which is closely associated with cancers. Therefore, developing detection technology for DNA MTase activity is significant for early diagnosis of cancer. Herein, a new and ultrasensitive photoelectrochemical (PEC) biosensor was fabricated to analyze DNA MTase activity using a dual-signal amplified system, where RGO-CdS:Mn nanoparticles and a CdTe@DNA network were used as photoelectric materials. RGO-CdS:Mn nanoparticles were first modified on a gold electrode as substrate materials. Then, probe DNA and target DNA with a recognition sequence for DNA methylation were assembled on the electrode. After the process of methylation and digestion, the methylated dsDNA could be.reserved and further hybridized with a CdTe@DNA network, leading to a stronger photocurrent. As expected, the PEC responses were in proportion to the logarithm of M.SssI MTase concentration from 0.01 to 80 U/mL with a detection limit of 0.0071 U/mL. This proposed method showed ideal detection ability for DNA MTase activity and could provide a new idea as well as technology in cancer diagnosis.