QUANTITATIVE-DETERMINATION OF LIGNANS AND ISOFLAVONOIDS IN PLASMA OF OMNIVOROUS AND VEGETARIAN WOMEN BY ISOTOPE-DILUTION GAS-CHROMATOGRAPHY MASS-SPECTROMETRY
QUANTITATIVE-DETERMINATION OF LIGNANS AND ISOFLAVONOIDS IN PLASMA OF OMNIVOROUS AND VEGETARIAN WOMEN BY ISOTOPE-DILUTION GAS-CHROMATOGRAPHY MASS-SPECTROMETRY
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DOI：
10.3109/00365519309090693
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发表时间：
1993-01-01
影响因子：
2.1
通讯作者：
HASE, T
中科院分区：
文献类型：
--
作者：
ADLERCREUTZ, H;FOTSIS, T;HASE, T
本文首次建立了血浆中木脂素类和甾体类植物雌激素的定量测定方法。使用离子交换色谱法，将二酚分离成两个部分：1）含有游离化合物+单硫酸盐和二硫酸盐的生物“活性"部分，和2）含有单葡萄糖醛酸苷和二葡萄糖醛酸苷以及磺基葡萄糖醛酸苷的生物”非活性"部分。水解后，通过固相萃取和离子交换色谱法进一步纯化级分。在第一步中使用放射性雌激素缀合物校正整个过程中的损失，随后通过加入所有测量化合物（马台树脂酚、肠二醇、肠内酯、大豆苷元、O-去甲基安哥拉辛、雌马酚和染料木黄酮）的氘代内标物校正。采用同位素稀释气相色谱-质谱法在选择离子监测模式（GC/MS/SIM）下进行最终测定。可以在低至0.2至1.0 nmol/l的浓度下测量二酚。27名绝经前后杂食和素食妇女的所有化合物的血浆分析结果首次提出。最重要的发现是染料木黄酮的游离+硫酸盐部分较低（占总量的3.8%），但肠内酯和肠二醇的含量高达21-25%。血浆和尿液值之间存在良好的相关性。个体化合物的总浓度在受试者之间差异很大（从pmol/l到mumol/l），素食者具有较高的值，特别是一个素食者受试者。最高总肠内酯浓度值超过1 mumol/l。得出结论，已开发出用于测定血浆中3种木脂素和4种木脂素的高度特异性方法。该方法为进一步研究木脂素和甘草酸的代谢提供了依据。
The first quantitative method for the determination of both lignans and isoflavonoid phytoestrogens in plasma is presented. Using ion-exchange chromatography the diphenols are separated into two fractions 1) the biologically ''active'' fraction containing the free compounds + mono- and disulfates and 2) the biologically ''inactive'' fraction containing the mono- and diglucuronides and the sulfoglucuronides. After hydrolysis the fractions are further purified by solid phase extraction and ion exchange chromatography. Losses during the complete procedure are corrected for using radioactive estrogen conjugates during the first steps and later by adding deuterated internal standards of all compounds measured (matairesinol, enterodiol, enterolactone, daidzein, O-desmethylangolensin, equol, and genistein). The final determination is carried out by isotope dilution gas chromatography-mass spectrometry in the selected ion monitoring mode (GC/MS/SIM). The diphenols may be measured at concentrations as low as 0.2 to 1.0 nmol/l. Results of plasma analyses of all compounds in 27 pre- and postmenopausal omnivorous and vegetarian women are presented for the first time. The most important findings are that the free+sulfate fraction is low for genistein (3.8% of total), but as much as 21-25% of enterolactone and enterodiol occurs in this fraction. A good correlation between plasma and urine values was found. Total concentrations of individual compounds vary greatly between the subjects (from pmol/l to mumol/l), the vegetarians having higher values, particularly one vegan subject. The highest total enterolactone concentration value exceeded 1 mumol/l. It is concluded that a highly specific method for the assay of 3 lignans and 4 isoflavonoids in plasma has been developed. This method will be useful in future studies of lignan and isoflavonoid metabolism..