Isolation of epidermal cells and cDNA cloning of TNF decoy receptor 3 of conger eel, Conger myriaster.
Isolation of epidermal cells and cDNA cloning of TNF decoy receptor 3 of conger eel, Conger myriaster.
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DOI：
10.1016/j.fsi.2007.12.001
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发表时间：
2008-03
影响因子：
4.7
通讯作者：
S. Tsutsui;Y. Yoshino;S. Matsui;O. Nakamura;K. Muramoto;Tasuku Watanabe
中科院分区：
文献类型：
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作者：
S. Tsutsui;Y. Yoshino;S. Matsui;O. Nakamura;K. Muramoto;Tasuku Watanabe
采用EDTA和胰蛋白酶溶液，建立了长鳝表皮细胞的分离方法。然后，我们从LPS刺激的表皮细胞制备的抑制减法杂交文库中鉴定了该物种的TNF诱饵受体（DcR） cDNA。较长的TNF - DcR （conDcR）全长cDNA由1479个碱基对组成，含有286个氨基酸残基。系统发育分析表明，conDcR属于DcR3分支。ConDcR可能作为一种重要的免疫调节因子，抑制长鳗皮肤TNF诱导细胞凋亡的作用。
By using EDTA and a trypsin solution, we established a method for isolating the epidermal cells of the conger eel, Conger myriaster. We then identified TNF decoy receptor (DcR) cDNA in the species from a suppression subtractive hybridization library prepared from the epidermal cells stimulated with LPS. The full-length cDNA of conger TNF DcR (conDcR) consisted of 1479 base pairs, and the protein comprised 286 amino acid residues. Phylogenetic analysis indicated that conDcR was clustered into a DcR3 branch. ConDcR is likely to act as an important immune-regulating factor in inhibiting the apoptosis-inducing effect of TNF in the skin of conger eel.