Immunity to Rice black streaked dwarf virus, a plant reovirus, can be achieved in rice plants by RNA silencing against the gene for the viroplasm component protein
Immunity to Rice black streaked dwarf virus, a plant reovirus, can be achieved in rice plants by RNA silencing against the gene for the viroplasm component protein
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DOI：
10.1016/j.virusres.2011.05.011
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发表时间：
2011-09-01
期刊：
影响因子：
5
通讯作者：
Sasaya, Takahide
中科院分区：
文献类型：
--
作者：
Shimizu, Takumi;Nakazono-Nagaoka, Eiko;Sasaya, Takahide
水稻黑条矮缩病毒的非结构蛋白P9-1已被证实在病毒囊中积累，病毒囊是病毒复制的假定位点，在受感染的植物和昆虫中。我们通过引入针对P9-1编码基因的RNA干扰构建体来转化水稻植物。所得转基因植物积累对构建体特异性的短干扰RNA。通过这些转基因植物的自交受精产生的所有后代都具有针对P9-1基因的诱导RNA干扰，对病毒的感染具有抗性。我们的研究结果表明，干扰植物呼肠孤病毒的病毒质组分蛋白的表达，这在病毒增殖中起着重要作用，可能是一种实用和有效的方法来控制植物呼肠孤病毒感染作物。(C)2011爱思唯尔有限公司版权所有。
The nonstructural protein P9-1 of Rice black streaked dwarf virus has been confirmed to accumulate in viroplasms, the putative sites of viral replication, in infected plants and insects. We transformed rice plants by introducing an RNA interference construct against the P9-1-encoding gene. The resultant transgenic plants accumulated short interfering RNAs specific to the construct. All progenies produced by self-fertilization of these transgenic plants with induced RNA interference against the gene for P9-1 were resistant to infection by the virus. Our results demonstrated that interfering with the expression of a viroplasm component protein of plant reoviruses, which plays an important role in viral proliferation, might be a practical and effective way to control plant reovirus infection in crop plants. (C) 2011 Elsevier B.V. All rights reserved.