HIGH-CELL-DENSITY CULTIVATION OF ESCHERICHIA-COLI

HIGH-CELL-DENSITY CULTIVATION OF ESCHERICHIA-COLI
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DOI:
10.1016/s0958-1669(05)80142-9
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发表时间:
1991-06-01
影响因子:
7.7
通讯作者:
RIESENBERG, D
RIESENBERG, D
中科院分区:
工程技术1区
文献类型:
--
作者:
RIESENBERG, D

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大肠杆菌在葡萄糖矿物盐培养基中的高细胞密度培养在仅进料葡萄糖和氨的特殊补料分批模式下产生超过100 g干细胞升-1。可以调节比生长速率以允许最佳重组蛋白产生。
High-cell-density cultivations of Escherichia coli in glucose-mineral-salt media produce more than 100 g dry cells litre-1 in special fed-batch modes with feeding of glucose and ammonia only. The specific growth rate can be adjusted to allow optimum recombinant protein generation.