Effect of Exogenous Nitro Oxide on Chilling Tolerance, Polyamine, Proline, and gamma-Aminobutyric Acid in Bamboo Shoots (Phyllostachys praecox f. prevernalis)
Effect of Exogenous Nitro Oxide on Chilling Tolerance, Polyamine, Proline, and gamma-Aminobutyric Acid in Bamboo Shoots (Phyllostachys praecox f. prevernalis)
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外源一氧化氮对竹笋 (Phyllostachys praecox f. prevernalis) 耐冷性、多胺、脯氨酸和 γ-氨基丁酸的影响
DOI：
10.1021/acs.jafc.7b02091
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发表时间：
2017
影响因子：
6.1
通讯作者：
Luo Zisheng
中科院分区：
文献类型：
--
作者：
Wang Di;Li Li;Xu Yanqun;Limwachiranon Jarukitt;Li Dong;Ban Zhaojun;Luo Zisheng
研究了外源一硝基（NO）对竹笋抗冷性及多胺、脯氨酸和γ-氨基丁酸代谢的影响。将竹笋浸泡在0.07mM硝普钠（SNP）中并在1 °C下储存56天。在贮藏过程中，SNP处理可抑制竹笋冷害的发展，降低丙二醛的积累和漏电。贮藏结束时，处理笋的冷害发生率比对照降低37.9%，丙二醛含量和漏电率分别降低8.8%和18.6%。处理后竹笋内源NO、多胺、γ-氨基丁酸和脯氨酸含量也显著增加。与此同时，一氧化氮合酶、精氨酸脱羧酶、鸟氨酸脱羧酶、谷氨酸脱羧酶、N-δ-氨基转移酶和Δ1-吡咯啉-5-羧酸合成酶等相关酶的活性均高于对照组（20.2%~ 49.8%），从而促进了竹笋含氮化合物的代谢。结果表明，SNP处理提高了竹笋的耐冷性，其机制可能与多胺、γ-氨基丁酸和脯氨酸的代谢活化有关。SNP处理可能是防止竹笋冷藏冷害的一种替代技术。
The effects of exogenous nitro oxide (NO) on chilling resistance and the metabolism of polyamine, proline, and γ-aminobutyric acid of bamboo shoots were investigated. Bamboo shoots were dipped in 0.07 mM sodium nitroprusside (SNP) and stored at 1 °C for 56 days. During the storage, the development of chilling injury of SNP treated bamboo shoots was inhibited with decreased accumulation of malonaldehyde and electrical leakage. At the end of storage, the chilling injury incidence of treated bamboo shoots decreased by 37.9% while their malonaldehyde content and electrical leakage were 8.8% and 18.6% lower than that of the control, respectively. Interestingly, the endogenous NO, polyamines, γ-aminobutyric acid, and proline contents of treated bamboo shoot also significantly increased. Consistently, the metabolisms of these nitrogenous compounds were stimulated in treated bamboo shoots, according to their higher (20.2%–49.8%) related enzyme activities, including nitric oxide synthase, arginine decarboxylase, ornithine decarboxylase, glutamate decarboxylase, orn-δ-aminotransferase, and Δ1-pyrroline-5-carboxylate synthetase. The results indicated that the SNP treatment enhanced chilling tolerance of bamboo shoots, which might associate with the activated metabolism of polyamines, γ-aminobutyric acid, and proline. SNP treatment might be an alternative technology to avoid chill injury during cold storage of bamboo shoots.