Transcription factor SOX2 up-regulates stomach-specific pepsinogen A gene expression
Transcription factor SOX2 up-regulates stomach-specific pepsinogen A gene expression
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DOI：
10.1007/s00432-006-0165-x
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发表时间：
2007-04-01
影响因子：
3.6
通讯作者：
Yuasa, Yasuhito
中科院分区：
文献类型：
--
作者：
Tani, Yasuyo;Akiyama, Yoshimitsu;Yuasa, Yasuhito
目的转录因子SOX 2在正常胃粘膜中表达，而在正常结肠中不表达。我们的目的是澄清SOX 2的作用与胃蛋白酶原表达在胃肠epithelium.Methods，我们分析了表达SOX 2和胃蛋白酶原，分化标志物的胃，在10个胃癌（GC）和10个结直肠癌（CRC）细胞系。结果RT-PCR检测到6株胃癌细胞和5株大肠癌细胞中有SOX 2的表达。在8个GC和7个CRC细胞系中可检测到胃蛋白酶原A的表达，而大多数细胞系表达胃蛋白酶原C。在293 T人胚肾细胞和一些GC和CRC细胞系中，SOX 2的过表达上调胃蛋白酶原A的表达，但不上调胃蛋白酶原C的表达。结论SOX 2在胃蛋白酶原A的表达调控中起重要作用，SOX 2的异位表达可能与大肠癌细胞的分化异常有关。
Purpose Transcription factor SOX2 is expressed in normal gastric mucosae but not in the normal colon. We aimed to clarify the role of SOX2 with reference to pepsinogen expression in the gastrointestinal epithelium.Methods We analyzed expression of SOX2 and pepsinogens, differentiation markers of the stomach, in ten gastric cancer (GC) and ten colorectal cancer (CRC) cell lines. The effects of over-expression and down-regulation of SOX2 on pepsinogen expression were also examined.Results Six GC and five CRC cell lines showed SOX2 expression on RT-PCR. Expression of pepsinogen A was detectable in eight GC and seven CRC cell lines, whereas the majority of the cell lines expressed pepsinogen C. Over-expression of SOX2 up-regulated expression of pepsinogen A but not that of pepsinogen C in 293T human embryonic kidney cells, and some GC and CRC cell lines. Moreover, pepsinogen A expression was significantly reduced by SOX2 RNA interference in two GC cell lines.Conclusions These data suggest that SOX2 plays an important role in regulation of pepsinogen A, and ectopic expression of SOX2 may be associated with abnormal differentiation of colorectal cancer cells.