PVA hydrogel sheet macroencapsulation for the bioartificial pancreas
PVA hydrogel sheet macroencapsulation for the bioartificial pancreas
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DOI：
10.1016/j.biomaterials.2004.01.050
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发表时间：
2004-12-01
期刊：
影响因子：
14
通讯作者：
Inoue, K
中科院分区：
文献类型：
--
作者：
Qi, M;Gu, Y;Inoue, K
1以含10%胎牛血清和5%二甲基亚砜的Euro-Collins液（PVA+EC）和3%聚乙烯醇（PVA）为囊液，采用冻融法制备了大鼠胰岛微囊。相同的包封技术，但3%的PVA仅溶解在双蒸水（PVA）和培养的游离胰岛作为对照。在CMRL-1066培养基中培养14天后，体外评价胰岛回收率、形态学变化、胰岛素含量和胰岛素分泌，以证明这种包封方法的可行性。我们还将该装置异种移植到糖尿病C57 BL/6小鼠的腹腔中，以检查其在体内的功能。培养1d后，PVA组胰岛回收率和胰岛素含量明显低于PVA + EC组和游离胰岛组。培养14 d后，只有PVA+EC组的胰岛仍保持正常形态和对高糖的有效胰岛素分泌反应，而PVA组在培养1 d后未观察到这种反应，而游离胰岛在培养7 d后也不再观察到这种反应。与假手术组相比，非空腹血糖水平从移植前的高血糖水平迅速下降，在整个研究过程中保持显著较低的血糖水平。结果表明，3%PVA + EC冻融微囊化技术是一种有效的胰岛细胞异种移植方法。(C)2004 Elsevier Ltd.保留所有权利。
lWe newly developed a sheet-type macroencapsulation device entrapping rat islets from 3% polyvinyl alcohol (PVA) dissolved in Euro-Collins solution containing 10% fetal bovine serum and 5% dimethyl sulfoxide (PVA+EC) using a freezing/thawing technique. The same encapsulation technique but with 3% PVA dissolved only in double-distilled water (PVA) and a culture of free islets were served as controls. After 14-day culture in the CMRL-1066 medium, the islet recovery rate, morphological changes, insulin content, and insulin secretion were evaluated in vitro to prove the feasibility of this method of encapsulation. We also xenotransplanted the device into the peritoneal cavity of diabetic C57BL/6 mice to check its function in vivo. After 1-day culture, the islet recovery rate and insulin content in the PVA group were significantly lower than that in the PVA + EC and free islet groups. After 14-day culture, only the islets in the PVA+EC group maintained a normal morphology and effective insulin secretory response to high glucose while the response was not observed in the PVA group after 1-day culture and no longer observed in the free islets after 7-day culture.After transplantation of rat islets encapsulated in the PVA + EC device to diabetic C57BL/6 mice, nonfasting blood glucose levels showed a rapid decrease from high glucose levels of pre-transplantation, maintaining significantly lower glucose levels during the whole course of study in comparison with the sham-operated group. Our results indicated that this freezing/thawing macroencapsulation technique using 3% PVA + EC was effective for xenotransplantation of islet cells. (C) 2004 Elsevier Ltd. All rights reserved.