Effects of immobilized recombinant human bone morphogenetic protein-2/succinylated type I atelocollagen on cellular activity of ST2 cells
Effects of immobilized recombinant human bone morphogenetic protein-2/succinylated type I atelocollagen on cellular activity of ST2 cells
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DOI：
10.1002/jbm.a.30416
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发表时间：
2005-10-01
影响因子：
4.9
通讯作者：
Nagai, N
中科院分区：
文献类型：
--
作者：
Tsujigiwa, H;Nagatsuka, H;Nagai, N
使用重组人骨形态发生蛋白-2(rhBMP-2)诱导异位成骨需要载体。I型人工晶状体是一种具有多孔结构、良好的操作性能和生物相容性的生物角膜材料，是重组人骨形态发生蛋白-2的有效载体。然而，传统使用的冷冻干燥的重组人骨形态发生蛋白-2/胶原混合物并不一定具有足够的骨诱导作用。在本研究中，我们检测了将重组人骨形态发生蛋白-2固定在I型胶原上对ST2细胞活性的影响。结果表明：(1)琥珀酸化的I型胶原能有效地固定化重组人骨形态发生蛋白-2，说明琥珀酸化的I型胶原具有良好的固定化效果；(2)碱性磷酸酶活性研究证实了琥珀酸化的胶原固定化的重组人骨形态发生蛋白-2具有增强细胞活性的作用。(C)2005年威利期刊公司。
The use of recombinant human bone morphogenetic protein-2 (rhBMP-2) to induce ectopic bone formation requires a carrier. Type I atelocollagen, a biornaterial with a porous structure, excellent operational features, and bio-compatibility, is an effective carrier for rhBMP-2. However, the conventionally used lyophilized rhBMP-2/collagen mixture does not necessarily give adequate bone-induction effect. In the present study, we examined the effect of immobilizing rhBMP-2 to type I atelocollagen on the cellular activity of ST2 cells. The following results were obtained: (1) rhBMP-2 was effectively immobilized to succinylated type I atelocollagen, indicating the usefulness of succinylated type I atelocollagen in immobilization; (2) studies of alkaline phosphatase activity confirmed the effectiveness of rhBMP-2 immobilized on succinylated atelocollagen in augmenting cellular activity. (c) 2005 Wiley Periodicals, Inc.