Identification of Veillonella Species in the Tongue Biofilm by Using a Novel One-Step Polymerase Chain Reaction Method.
Identification of Veillonella Species in the Tongue Biofilm by Using a Novel One-Step Polymerase Chain Reaction Method.
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DOI：
10.1371/journal.pone.0157516
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Nakazawa F
中科院分区：
文献类型：
--
作者：
Mashima I;Theodorea CF;Thaweboon B;Thaweboon S;Nakazawa F
六种韦荣氏球菌经常从人类口腔包括感染部位分离。最近，据报道，饮食，吸烟和可能的社会经济地位可以影响口腔中的细菌分布。此外，口腔卫生习惯也可能在微生物的数量和多样性方面影响口腔微生物群。在这项研究中，鉴定韦荣氏菌属物种的舌头生物膜的泰国儿童，分为三组，取决于他们的口腔卫生状况。为此，我们使用了一种新的一步PCR方法，该方法具有基于rpoB基因序列的物种特异性引物组。如结果所示，韦荣球菌属的分离株数量为101株，仅来自89名受试者中的10名。然而，所有受试者的细菌总数都很高。由于在以前的研究中报告说，韦荣球菌属物种很容易在人类舌生物膜中分离出大量，因此本研究中获得的结果可能表明国家或年龄特异性差异。此外，韦荣氏球菌物种主要是在口腔卫生不良的受试者中检测到的，而口腔卫生良好或中等的受试者。从这些结果来看，韦荣氏球菌可能是口腔卫生状况的一个指标。此外，在泰国儿童的舌生物膜中，Rogosae弧菌是优势种，而小弧菌和齿垢弧菌根本没有分离出来。这些特征的分布和频率的韦荣球菌属物种是在以前的研究报告相似。虽然在其他国家还需要进一步的研究，在本研究中，成功地建立了一个新的一步PCR方法，以检测人口腔中的韦荣球菌物种容易和有效的。此外，这是第一份报告调查的分布和频率韦荣氏球菌物种的舌头生物膜的泰国儿童。
Six Veillonella species have been frequently isolated from human oral cavities including infectious sites. Recently, it was reported that diet, smoking, and possibly socioeconomic status can influence the bacterial profile in oral cavities. In addition, oral hygiene habits may also influence oral microbiota in terms of both numbers and diversity of microorganisms. In this study, the identification of Veillonella species in tongue biofilms of Thai children, divided into three groups dependent on their status of oral hygiene. For this, we used a novel one-step PCR method with species-specific primer sets based on sequences of the rpoB gene. As shown in the results, the number of isolates of Veillonella species was 101 strains from only 10 of 89 subjects. However, the total number of bacteria was high for all subjects. Since it was reported in previous studies that Veillonella species were easy to isolate in human tongue biofilms at high numbers, the results obtained in this study may suggest country- or age-specific differences. Moreover, Veillonella species were detected predominantly in subjects who had poor oral hygiene compared to those with good or moderate oral hygiene. From these results, there is a possibility that Veillonella species may be an index of oral hygiene status. Furthermore, V. rogosae was a predominant species in tongue biofilms of Thai children, whereas V. parvula and V. denticariosi were not isolated at all. These characteristics of the distribution and frequency of Veillonella species are similar to those reported in previous studies. Although further studies are needed in other countries, in this study, a successful novel one-step PCR method was established to detect Veillonella species in human oral cavities easily and effectively. Furthermore, this is the first report investigating the distribution and frequency of Veillonella species in tongue biofilms of Thai children.