Examination of bacterial flagellation by dark-field microscopy
Examination of bacterial flagellation by dark-field microscopy
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用暗视野显微镜检查细菌鞭毛
DOI：
10.1128/jcm.4.3.258-265.1976
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发表时间：
1976
影响因子：
9.4
通讯作者：
R. Macnab
中科院分区：
文献类型：
--
作者：
R. Macnab
描述了一种通过暗视野光学显微镜观察未染色细菌鞭毛的方法。由于可以看到单独的丝状体，因此可以很容易地将诸如沙门氏菌属的周毛鞭毛与诸如假单胞菌属的极鞭毛区分开来。极性鞭毛细菌通常比周毛鞭毛细菌游得快得多，并通过突然的逆转而转向。鞭毛和运动性的差异提供了可能在临床微生物学中有用的诊断标准。
A method is described for visualizing unstained bacterial flagella by dark-field light microscopy. Since individual filaments can be seen, a genus such as Salmonella, which is peritrichously flagellated, can readily be distinguished from a polarly flagellated genus such as Pseudomonas. Polarly flagellated bacteria generally swim much faster than peritrichously flagellated bacteria, and turn by abrupt reversals. The differences in flagellation and motility provide diagnostic criteria that may be useful in clinical microbiology.