Assessment of the genetic connectivity between farmed and wild populations of Undaria pinnatifida (Phaeophyceae) in a representative traditional farming region of China by using newly developed microsatellite markers
Assessment of the genetic connectivity between farmed and wild populations of Undaria pinnatifida (Phaeophyceae) in a representative traditional farming region of China by using newly developed microsatellite markers
复制标题
利用新开发的微卫星标记评估中国代表性传统农耕区养殖和野生裙带菜（褐藻纲）种群之间的遗传连通性
DOI：
10.1007/s10811-018-1449-7
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发表时间：
2018
影响因子：
3.3
通讯作者：
Su Li
中科院分区：
文献类型：
--
作者：
Shan Tifeng;Pang Shaojun;Wang Xuemei;Li Jing;Su Li
裙带菜是东亚地区广泛种植的重要经济作物。美国野生个体。由于其特殊的形态，可以很容易地辨别出羽裂，通常观察到羽裂发生在耕作区中与耕作的基础设施相邻的耕作基础设施上。然而，野生和养殖种群之间的遗传联系仍然没有确定。在这项研究中，30个信息微卫星标记的开发和表征，通过下一代测序技术。利用其中的10个引物对2015 - 2017年在旅顺（北纬38 ° 47 ′）一个具有代表性的裙带菜养殖场采集的野生和养殖群体进行遗传结构分析，并以青岛（北纬36 ° 03 ′）和枸杞岛（北纬30 ° 42 ′）的2个野生群体作为对照。聚类图清楚地将养殖群体与野生群体分开，遗传距离很大。基于贝叶斯模型的结构分析将所有人口分为五个聚类。在农场的野生个体被发现包含相对较大比例的成员来自养殖的。与此相反，几乎没有野生成员被发现在养殖种群。这些结果意味着不对称的基因流之间的养殖和野生种群，并表明，养殖品种的谱系已被很好地保持通过人工控制，在目前的种苗生产系统。应大力推广该系统，以利于裙带菜栽培品种的可持续利用。
Undaria pinnatifida is an important economic macroalga extensively farmed in East Asia. Wild individuals of U. pinnatifida, which can be easily discriminated due to their peculiar morphology, are usually observed to occur on the cultivation infrastructure adjacent to the farmed ones in the farming area. The genetic connectivity between the wild and farmed populations, however, remains unidentified. In this study, 30 informative microsatellite markers were developed and characterized through the next-generation sequencing technology. Ten of them were used to analyze the genetic structure of wild and farmed populations sampled from a representative Undaria farm in Lüshun (latitude: 38° 47′ N) from 2015 to 2017, with two wild populations from Qingdao (36° 03′ N) and Gouqi Island (30° 42′ N) as controls. The dendrogram clearly separated farmed populations from wild ones by large genetic distance. The Bayesian model-based structure analysis grouped all the populations into five clusters. The wild individuals on the farm were found to contain relatively a large proportion of membership originated from farmed ones. In contrast, nearly no wild membership was detected in the farmed populations. These results imply asymmetric gene flow between farmed and wild populations and indicate that the pedigree of farmed cultivars has been well maintained through artificial control in the current seedling production system. Efforts should be made to popularize this system for the benefits of sustainable utilization of cultivars in Undaria farming.