Phylogenetic analysis of the human ancient mitochondrial DNA
Phylogenetic analysis of the human ancient mitochondrial DNA
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DOI：
10.1016/j.jas.2004.02.011
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发表时间：
2004-10-01
影响因子：
2.8
通讯作者：
Sakaue, K
中科院分区：
文献类型：
--
作者：
Adachi, N;Umetsu, K;Sakaue, K
为了确定从考古遗址发现的人类骨骼遗骸的家谱，我们详细分析了线粒体DNA(MtDNA)的编码区。这一地区既有重要的家谱，又相对不受死后损害的影响。我们还对mtDNA控制区的片段进行了测序，并使用已知的mtDNA数据库将研究中的mtDNA分配到相关单倍群。之后，我们根据控制区和编码区的序列多态将每个mtDNA划分为母系。这种方法对古代DNA分析特别有用，因为它使我们能够高度确定个人的家谱。因此，可以更清楚、更可靠地推断古代个体的遗传结构、起源和时间变化。此外，结果的错误重组是mtDNA片段分析的主要问题，可以通过使用单群基序分析来寻找结果之间的不一致性来监视结果的错误重组。(C)2004爱思唯尔有限公司。保留所有权利。
To identify the genealogy of human skeletal remains recovered from an archaeological site, we analyzed the coding region of mitochondrial DNA (mtDNA) in detail. This region is both genealogically important, and relatively impervious to postmortem damage. We also sequenced the segments of the control regions of mtDNA, and assigned the mtDNA under study to relevant haplogroups using the known mtDNA database. Thereafter, we classified each mtDNA into maternal lines according to the sequence polymorphism of the control and coding region. This approach is especially useful for ancient DNA analysis, because it enables us to identify the genealogy of the individuals with high level of certainty. Therefore, the genetic structure of the ancient individuals, their origins and temporal changes can be inferred more clearly and reliably. Moreover, erroneous recombination of the results, which is the major problem of the segmental analysis of mtDNA, can be monitored by looking out for the incongruence between the results by using haplogroup motif analysis. (C) 2004 Elsevier Ltd. All rights reserved.