Measurement of lipid forces by X-ray diffraction and osmotic stress.
Measurement of lipid forces by X-ray diffraction and osmotic stress.
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通过 X 射线衍射和渗透应力测量脂质力。
DOI：
10.1007/978-1-59745-519-0_27
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Parsegian,VAdrian
中科院分区：
文献类型：
--
作者：
Petrache,HoriaI;Harries,Daniel;Parsegian,VAdrian
水溶液中的脂类悬浮液通常形成均匀分布的双层的多层囊泡。片层间距由吸引的范德华(电荷波动)和排斥力的平衡决定。这种力的平衡，以及膜的弹性，可以通过施加渗透应力来探测。我们描述了如何对多层脂质样本施加渗透压力来研究脂质相互作用。
Lipid suspensions in aqueous solutions most often form multilamellar vesicles of uniformly spaced bilayers. Interlamellar spacing is determined by the balance of attractive van der Waals (charge fluctuation) and repulsive forces. This balance of forces, as well as membrane elasticity, can be probed by applied osmotic stress. We describe how osmotic stress can be imposed on multilamellar lipid samples to study lipid interactions.