Induction of shikimate dehydrogenase and peroxidase in pepper (Capsicum annuum L.) seedlings in response to copper stress and its relation to lignification
Induction of shikimate dehydrogenase and peroxidase in pepper (Capsicum annuum L.) seedlings in response to copper stress and its relation to lignification
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DOI：
10.1016/s0168-9452(01)00410-1
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发表时间：
2001-06-01
期刊：
影响因子：
5.2
通讯作者：
Merino, F
中科院分区：
文献类型：
--
作者：
Díaz, J;Bernal, A;Merino, F
辣椒（Capsicum annuum L.）在含有过量铜的营养液中生长的植物显示下胚轴中莽草酸脱氢酶（SKDH，ECwww.example.com）和过氧化物酶（1.11.1.7）活性增加。1.1.1.25在根中，过氧化物酶也被诱导，但每个器官的SKDH活性被耗尽，而不是增强。铜胁迫导致植物生长迟缓，反映在所有器官的鲜重下降。在下胚轴中，这两种酶活性的诱导与可溶性酚类物质和木质素的积累有关。铜胁迫后，对照下胚轴（SKDH-3和SKDH-4）中存在的两种SKDH同工酶以相似的比例增加。在过氧化物酶方面，在铜胁迫下，下胚轴中检测到两种新的同工酶（PRX-A2和PRX-A4），另外两种同工酶（PRX-B和PRX-A3）在铜胁迫下表达增强。10倍和3倍。螯合剂EDTA的应用能够抵消上述金属的所有应力效应。这些酶的作用，在酚类代谢重金属胁迫下进行了讨论。(C)2001爱思唯尔科学爱尔兰有限公司保留所有权利。
Pepper (Capsicum annuum L.) plants growing in a nutrient solution with excess copper, showed an increase in shikimate dehydrogenase (SKDH, EC 1.1.1.25) and peroxidase (EC 1.11.1.7) activities in the hypocotyl. In the roots, peroxidase was also induced, but SKDH activity per organ was depleted rather than enhanced. Copper stress caused stunting in the plants, reflected by a decrease in the fresh weight of all the organs. In the hypocotyl, the induction of both enzymatic activities was associated with the accumulation of soluble phenolics and lignin. The two SKDH isozymes present in the control hypocotyls (SKDH-3 and SKDH-4) increased in a similar proportion after copper stress. In the case of peroxidases, two new isozymes (PRX-A2 and PRX-A4) were detected in copper-stressed hypocotyls, and the other two isoperoxidases, PRX-B and PRX-A3, were enhanced c. 10 and three times, respectively, with respect to the control. The application of the chelator EDTA was able to counteract all the stress effects of the metal cited above. The role of these enzymes in phenolic metabolism under heavy metal stress is discussed. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.