A strategic sampling design revealed the local genetic structure of cold-water fluvial sculpin: a focus on groundwater-dependent water temperature heterogeneity
A strategic sampling design revealed the local genetic structure of cold-water fluvial sculpin: a focus on groundwater-dependent water temperature heterogeneity
复制标题
战略抽样设计揭示了冷水河流杜父鱼的局部遗传结构：关注地下水依赖的水温异质性
DOI：
10.1038/s41437-021-00468-z
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发表时间：
2021
期刊：
影响因子：
3.8
通讯作者：
Nakamura Futoshi
中科院分区：
文献类型：
--
作者：
Nakajima Souta;Sueyoshi Masanao;Hirota Shun K.;Ishiyama Nobuo;Matsuo Ayumi;Suyama Yoshihisa;Nakamura Futoshi
生态系统管理的一个关键信息是环境与种群遗传结构之间的关系。然而，由于空间自相关和分析问题，环境因子对遗传分化的影响难以明确量化。在本研究中，我们以地下水引起的河流生态系统和环境异质性为研究对象，构建了地理距离和环境差异不相关的采样设计。采用多路ISSR基因分型测序（MIG-seq）方法，对河流斑纹棉（cottus nozawae）进行了精细种群遗传学研究，夏季水温是影响其分布和生存的决定性因素。在流域中有一个清晰的遗传结构。虽然在流域中发现了显著的距离隔离模式，但遗传分化与水温之间没有关联。相反，检测到从相对低温溪流到高温溪流的不对称基因流动，表明低温溪流和连续栖息地的重要性。以地下水为中心的采样策略产生了深刻的保护结果。
A key piece of information for ecosystem management is the relationship between the environment and population genetic structure. However, it is difficult to clearly quantify the effects of environmental factors on genetic differentiation because of spatial autocorrelation and analytical problems. In this study, we focused on stream ecosystems and the environmental heterogeneity caused by groundwater and constructed a sampling design in which geographic distance and environmental differences are not correlated. Using multiplexed ISSR genotyping by sequencing (MIG-seq) method, a fine-scale population genetics study was conducted in fluvial sculpinCottus nozawae, for which summer water temperature is the determinant factor in distribution and survival. There was a clear genetic structure in the watershed. Although a significant isolation-by-distance pattern was detected in the watershed, there was no association between genetic differentiation and water temperature. Instead, asymmetric gene flow from relatively low-temperature streams to high-temperature streams was detected, indicating the importance of low-temperature streams and continuous habitats. The groundwater-focused sampling strategy yielded insightful results for conservation.