Gene switching and the stability of odorant receptor gene choice
Gene switching and the stability of odorant receptor gene choice
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DOI：
10.1016/j.cell.2004.05.015
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发表时间：
2004-06-11
期刊：
影响因子：
64.5
通讯作者：
Barnea, G
中科院分区：
文献类型：
--
作者：
Shykind, BM;Rohani, SC;Barnea, G
单个嗅觉感觉神经元只表达来自大家族基因的单一气味受体，这种独特性是嗅觉感知模型的基本特征。我们设计了一种遗传策略来检验受体选择的稳定性。我们观察到，表达给定气味受体的未成熟嗅觉感觉神经元可以转换受体表达，尽管频率很低。表达突变受体基因的神经元更有可能切换受体转录，这表明功能性气味受体的表达引发了一个反馈信号，终止了开关（Lewcock和Reed, 2004; Serizawa et al., 2003）。这种受体基因转换的过程保证了神经元最终会表达一种功能性受体，并且这种受体的选择将在细胞的生命周期中保持稳定。
Individual olfactory sensory neurons express only a single odorant receptor from a large family of genes, and this singularity is an essential feature in models of olfactory perception. We have devised a genetic strategy to examine the stability of receptor choice. We observe that immature olfactory sensory neurons that express a given odorant receptor can switch receptor expression, albeit at low frequency. Neurons that express a mutant receptor gene switch receptor transcription with significantly greater probability, suggesting that the expression of a functional odorant receptor elicits a feedback signal that terminates switching (Lewcock and Reed, 2004; Serizawa et al., 2003). This process of receptor gene switching assures that a neuron will ultimately express a functional receptor and that the choice of this receptor will remain stable for the life of the cell.