A method for quantifying Langerhans cells in epidermal sheets of human and murine skin.
A method for quantifying Langerhans cells in epidermal sheets of human and murine skin.
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一种定量人和鼠皮肤表皮层中朗格汉斯细胞的方法。
DOI：
10.1111/j.1399-0039.1981.tb00686.x
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
A. Ackles
中科院分区：
文献类型：
--
作者：
J. Nordlund;A. Ackles
朗格汉斯细胞是位于表皮的树突状细胞。先前已使用多种技术对与真皮分离的表皮片中的这些细胞进行染色。 ATP酶技术是最常用的，但有几个显着的缺陷，最重要的是它不稳定且非特异性。朗格汉斯细胞和不确定细胞是表皮中唯一表面具有Ia抗原的树突状细胞。我们采用了间接免疫荧光方法来对表皮片中的这组细胞进行染色。该技术灵敏、特异，可用于识别和定量人类或小鼠表皮片中的朗格汉斯细胞。
Langerhans cells are dendritic cells located in the epidermis. Several techniques have been used previously to stain these cells in sheets of epidermis separated from dermis. The ATPase technique is most commonly used but has several significant defects, the most important being that it is inconstant and nonspecific. Langerhans cells and indeterminate cells are the only dendritic cells in the epidermis which have Ia antigens on their surface. We have adapted an indirect immunofluorescence method to stain this population of cells in epidermal sheets. The technique is sensitive, specific, and can be used to identify and quantify Langerhans cells in human or murine epidermal sheets.