Histological characteristics of peri-implant mucosa around Branemark and single-crystal sapphire implants
Histological characteristics of peri-implant mucosa around Branemark and single-crystal sapphire implants
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DOI：
10.1034/j.1600-0501.1996.070101.x
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发表时间：
1996-03-01
影响因子：
4.3
通讯作者：
Kondell, PA
中科院分区：
文献类型：
--
作者：
Arvidson, K;Fartash, B;Kondell, PA
通过传统光学和透射电子显微镜以及细胞角蛋白、蛋白质 S-100、因子 VIII 和 KP1 的免疫组织化学标记物研究了 Branemark 钛植入物和单晶蓝宝石植入物周围的软组织。从临床健康的种植体周围粘膜获得的活检组织学切片被分成角化的外部种植体上皮和内部的非角化上皮，两者都对细胞角蛋白具有免疫反应性。内部种植体上皮终止于连接上皮，顶部只有几个细胞层厚。与植入物相邻的细胞显示出浓缩的细胞质，类似于半桥粒。在富含成纤维细胞和因子 VIII 免疫反应血管的底层结缔组织中，胶原蛋白束朝不同的方向延伸。 S-100 免疫反应性神经结构更常见于外部种植体上皮下方，而不是内部种植体上皮下方。在内种植体上皮的顶端部分观察到炎症细胞浸润，部分 KP1 呈阳性。 S-100 阳性朗格汉斯细胞主要存在于外种植体上皮内。对于这两种植入系统，这些技术揭示了相邻软组织中没有性质上的结构差异。
Soft tissues surrounding Branemark titanium implants and single crystal sapphire implants were studied by conventional light- and transmission electron microscopy and by immunohistochemical markers for cytokeratin, protein S-100, Factor VIII and KP1. Histological sections of biopsies obtained from clinically healthy peri-implant mucosa were separated into a keratinized outer implant epithelium and an inner, non-keratinized epithelium, both immunoreactive towards cytokeratin. The inner implant epithelium terminated in a junctional epithelium, apically only a few cell layers thick. The cells adjacent to the implant showed a condensed cytoplasm, resembling hemidesmosomes. In the underlying connective tissue, rich in fibroblasts and factor VIII immunoreactive blood vessels, the bundles of collagen ran in different directions. S-100 immunoreactive nerve structures were more frequently found beneath the outer than the inner implant epithelium. Inflammatory cell infiltrates, some KP1 positive, were observed in the apical parts of the inner implant epithelium. S-100 positive Langerhans' cells were present mainly within the the outer implant epithelium. For the two implant systems, the techniques disclosed no qualitative structural differences in the adjacent soft tissues.